Environmental Factors Preceding A40 Monomer to Oligomers and the Detection of Oligomers in Alzheimer's Disease Patient Serum
Identification of response of temperature-modified serum of patients with Alzheimer’s disease . According to the criteria of National Institute of Neurological and Communicative Disorders and Stroke/Alzheimer’s Disease and Related Disorders, AD patients were enrolled, and the patients were classified into two groups of mild AD and severe AD with age match. MMSE score 1–9 was as severe AD and the score of 10–22 was as mild AD. Sera from two AD groups were tested for their reactivity against 4G8 with ELISA. (a) The absorbance in the severe AD was lower than that of the mild AD though the temperatures and the signals from mild AD were almost constant; however, the significant lower signal was observed in severe AD at 40°C () and 41°C (). The signals from Aβ40 peptide as a control showed decrease as the temperature increase in a temperature-dependent manner. (b) The minimum/maximum optical density ratio of each patient’s serum was calculated and obtained the average of ratios for severe and mild AD. The ratio value for patients with severe AD () was significantly () lower than that for patients with mild AD (). The average min/max optical density value for the synthetic Aβ1–40 peptide, used as a control, was .
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