Elucidation of the Rotavirus NSP4-Caveolin-1 and -Cholesterol Interactions Using Synthetic Peptides
Figure 9
Cholesterol directly binds NSP4112-140 peptide. (a) Fluorescence spectra of Cy5-NSP4112-140 titrated with increasing concentrations of cholesterol. Spectrum 1 : 10 nM Cy5-NSP4112-140 in buffer only. Spectra 2–8 : 10 nM Cy5-NSP4112-140 in the presence of 5, 10, 15, 20, 25, 20 and 35 nM of cholesterol, respectively. (b) Plot of maximal fluorescence emission (measured at 665 nm upon excitation at 649 nm) for cholesterol in the presence of 10 nM Cy5-NSP4112-140. Values represent the mean ± S.E., . (c) Fluorescence spectra of Cy5-NSP4150-175ΔAla (negative control) titrated with increasing concentrations of cholesterol. (d) Plot of maximal fluorescence emission (measured at 665 nm upon excitation at 649 nm) for cholesterol in the presence of 10 nM Cy5-NSP4150-175ΔAla. Values represent the mean ± S.E., .
