Elucidation of the Rotavirus NSP4-Caveolin-1 and -Cholesterol Interactions Using Synthetic Peptides

Table 4

Observed and theoretical % helical content of NSP4_112-140-caveolin-1 peptide-peptide interactions.

	N-Cav2-20		Cav19-40		Cav68-80		C-Cav161-178
	Observed	Theoretical	Observed	Theoretical	Observed	Theoretical	Observed	Theoretical
Aqueous buffer	21.7±0.8	23.6±2.6	23.8±1.2	24.4±0.6	24.2±0.3	23.9±0.7	25.0±1.7 *	35.3±1.6
50% TFE	46.3±7.2	47.6±1.8	58.7±7.7	56.3±2.3	45.9±2.8	49.8±1.8	44.2±10.6 *	67.8±3.2
1 mM SUV	34.7±6.0	37.7±1.9	37.7±3.6	39.0±2.3	31.3±0.2 *	37.5±1.9	51.9±2.4	49.5±6.2

NSP4_112-140-Caveolin-1 Peptide-Peptide Interactions: Percent α-helical content of NSP4_112-140 peptide + Caveolin-1 peptides, in aqueous buffer (10 mM potassium phosphate buffer), 50% trifluoroethanol (TFE) and in the presence of 1 mM SUV (55 : 35 : 10, POPC/cholesterol/DOPS). Observed values were compared with theoretical values, and those that were statistically significant by a Student’s 𝑡-test (𝑃<0.05; denoted by an asterisk) were considered structurally altered, indicative of an interaction. Results are presented as means ± S.D. (𝑛=3 or 4).