Role of Linkers between Zinc Fingers in Spacing Recognition by Plant TFIIIA-Type Zinc-Finger Proteins
Figure 2
SAAB-imprinting assay of ZPT2-14-binding sequences. (a, b), Gel mobility shift assays after the first (a) and the fifteenth (b) rounds of selection by SAAB-imprinting procedure for ZPT2-14-binding sequence. Concentrations of ZPT2-14ZF in the reaction mixture are shown at the top of the lanes. Oligonucleotides were recovered from the shifted bands indicated by rectangles and processed. (c), Enrichment of respective triplets after 15 rounds of selection. Shown are increases in total numbers of each triplet occurring on both strands over those in the sequences before selection. (d), Enrichment of tetramers after 15 rounds of selection. Shown are increases in total numbers of tetramers including AGT or GTG over those in the sequences before selection.
