Role of Linkers between Zinc Fingers in Spacing Recognition by Plant TFIIIA-Type Zinc-Finger Proteins
Figure 6
Recognition of spacing in target sequences. (a)–(d), DNA binding of four ZPT peptides—ZPT2-14ZF (a), ZPT2-7ZFB (b), ZPT2-8ZFB (c), and ZPT2-2ZF (d)—to DNA sequences with various lengths of spacing between two core sequences. Fractions of bound DNA are shown with representative electrophoretic patterns above them. The data are averages of four experiments, with bars representing standard deviation. (e) Sequences of the central regions of probes. Two core binding sites (AGT) in each probe are underlined. Besides the core sites, AGT/ACT is excluded from these regions, and AT/GC contents were balanced in these regions. Sequences flanking the central regions are 5′-CTCGAGAAATTCC-central region-GGCCGGGGGGATCC-3′.
