Table of Contents
Journal of Amino Acids
Volume 2013 (2013), Article ID 569527, 6 pages
http://dx.doi.org/10.1155/2013/569527
Research Article

Peptide Mass Fingerprinting and N-Terminal Amino Acid Sequencing of Glycosylated Cysteine Protease of Euphorbia nivulia Buch.-Ham.

1Department of Biotechnology, Faculty of Science, Post Graduate College of Science, Technology and Research, North Maharashtra University, Jalgaon, Maharashtra 425002, India
2Department of Biotechnology, Faculty of Science, Moolji Jaitha College, Jalgaon, Maharashtra 425002, India

Received 24 October 2012; Revised 30 December 2012; Accepted 30 December 2012

Academic Editor: Hieronim Jakubowski

Copyright © 2013 Shamkant B. Badgujar and Raghunath T. Mahajan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. T. Mahajan and S. B. Badgujar, “Biological aspects of proteolytic enzymes: a review,” Journal of Pharmacy Research, vol. 3, no. 9, pp. 2048–2068, 2010. View at Google Scholar
  2. N. M. Rawlings and A. J. Barrett, Handbook of Proteolytic Enzymes, vol. 2, Academic Press, London, UK, 2nd ed edition, 2004.
  3. C. Sequeiros, M. J. Torres, S. A. Trejo, J. L. Esteves, C. L. Natalucci, and L. M. I. López, “Philibertain g I, the most basic cysteine endopeptidase purified from the latex of Philibertia gilliesii hook. et Arn. (Apocynaceae),” The Protein Journal, vol. 24, no. 7-8, pp. 445–453, 2005. View at Publisher · View at Google Scholar · View at Scopus
  4. V. Kumar Dubey and M. V. Jagannadham, “Procerain, a stable cysteine protease from the latex of Calotropis procera,” Phytochemistry, vol. 62, no. 7, pp. 1057–1071, 2003. View at Publisher · View at Google Scholar · View at Scopus
  5. S. B. Badgujar and R. T. Mahajan, “Comparison of cysteine proteases of four laticiferous plants and characterization of Euphorbia nivulia Buch.-Ham. latex glycosylated cysteine peptidase,” Indian Journal of Natural Products and Resources, vol. 3, no. 2, pp. 152–160, 2012. View at Google Scholar
  6. R. T. Mahajan and S. B. Badgujar, “Bioprospecting of Euphorbia nivulia Buch.-Ham.,” International Journal of Phytopharmacology, vol. 2, no. 2, pp. 37–42, 2011. View at Google Scholar
  7. S. B. Badgujar and R. T. Mahajan, “Characterization of milk clotting cysteine protease of Euphorbia nivulia Buch.-Ham. latex,” Green Farming, vol. 1, no. 6, pp. 645–648, 2010. View at Google Scholar
  8. S. B. Badgujar and R. T. Mahajan, “Characterization of 31-kDa, Tubulin alpha-1 chain like protein of Euphorbia nivulia Buch.-Ham. latex,” Biochemistry: An Indian Journal, vol. 5, no. 4, pp. 258–262, 2011. View at Google Scholar
  9. S. L. Cohen and B. T. Chait, “Mass spectrometry of whole proteins eluted from sodium dodecyl sulfate- polyacrylamide gel electrophoresis gels,” Analytical Biochemistry, vol. 247, no. 2, pp. 257–267, 1997. View at Publisher · View at Google Scholar · View at Scopus
  10. X. Wang, M. Shi, X. Lu et al., “A method for protein extraction from different subcellular fractions of laticifer latex in Hevea brasiliensis compatible with 2-DE and MS,” Proteome Science, vol. 8, article 35, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. S. F. Altschul, T. L. Madden, A. A. Schäffer et al., “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,” Nucleic Acids Research, vol. 25, no. 17, pp. 3389–3402, 1997. View at Publisher · View at Google Scholar · View at Scopus
  12. S. B. Badgujar, Proteolytic enzymes of some latex bearing plants belonging to Khandesh region of Maharashtra [Ph.D. thesis], North Maharashtra University, Jalgaon (Maharashtra State), India, 2011.
  13. S. R. Morcelle, S. A. Trejo, F. Canals, F. X. Aviles, and N. S. Priolo, “Funastrain c II: a cysteine endopeptidase purified from the latex of Funastrum clausum,” Protein Journal, vol. 23, no. 3, pp. 205–215, 2004. View at Publisher · View at Google Scholar · View at Scopus
  14. K. H. Choi and R. A. Laursen, “Amino-acid sequence and glycan structures of cysteine proteases with proline specificity from ginger rhizome Zingiber officinale,” European Journal of Biochemistry, vol. 267, no. 5, pp. 1516–1526, 2000. View at Publisher · View at Google Scholar · View at Scopus
  15. J. B. Bouwstra, E. C. Spoelstra, P. De Waard, B. R. leeflang, J. P. Kamerling, and J. F. G. Vliegenthart, “Conformational studies on the N-linked carbohydrate chain of bromelain,” European Journal of Biochemistry, vol. 190, no. 1, pp. 113–122, 1990. View at Publisher · View at Google Scholar · View at Scopus
  16. P. Pinlaor, N. Kaewpitoon, T. Laha et al., “Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini,” PLoS Neglected Tropical Diseases, vol. 3, no. 3, article no. e398, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. T. Nikawa, T. Towatari, and N. Katunuma, “Purification and characterization of cathepsin J from rat liver,” European Journal of Biochemistry, vol. 204, no. 1, pp. 381–393, 1992. View at Google Scholar · View at Scopus
  18. U. Von Pawel-Rammingen, B. P. Johansson, and L. Björck, “IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G,” The EMBO Journal, vol. 21, no. 7, pp. 1607–1615, 2002. View at Publisher · View at Google Scholar · View at Scopus
  19. S. Q. Peng, J. H. Zhu, H. L. Li, and W. M. Tian, “Cloning and characterization of a novel cysteine protease gene (HbCP1) from Hevea brasiliensis,” Journal of Biosciences, vol. 33, no. 5, pp. 681–690, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. L. K. Fritz-Laylin, S. E. Prochnik, M. L. Ginger et al., “The genome of Naegleria gruberi illuminates early eukaryotic versatility,” Cell, vol. 140, no. 5, pp. 631–642, 2010. View at Publisher · View at Google Scholar · View at Scopus