Total 4EBP1 Is Elevated in Liver of Rats in Response to Low Sulfur Amino Acid Intake

Table 1

Composition of experimental diets.

	Control
	0.23% M/0.35% C*	0.11% M/0.35% C	0.23% M	0.11% M
Ingredient	g/kg diet
L-Amino acid mix^†	172	172	172	172
L-Methionine	2.3	1.1	2.3	1.1
L-Cystine	3.5	3.5	0	0
Cornstarch	389.9	389.9	389.9	389.9
Dextrinized corn starch	155	155	155	155
Sucrose	102.4	103.6	105.9	107.1
Cellulose	50	50	50	50
Soybean oil	70	70	70	70
tert-Butylhydroquinone	0.01	0.01	0.01	0.01
Mineral mix	35	35	35	35
Vitamin mix	10	10	10	10
Choline bitartrate	2.5	2.5	2.5	2.5
Sodium bicarbonate	7.4	7.4	7.4	7.4
Methionine equivalents,; g/kg^‡	6.6	5.4	2.3	1.1

∗M: L-methionine, C: L-cystine.
^†L-amino acid mix (g/kg): L-arginine 6.3, L-histidine 4.5, L-tyrosine 9.2, L-phenylalanine 8.7, L-leucine 15.3, L-Isoleucine 8.4, L-valine 9.9, glycine 3.1, L-proline 20.4, L-glutamic acid 36.2, L-alanine 4.5, L-aspartic acid 11.3, L-serine 9.4, L-lysine-HCl 16.1, L-threonine 6.6, and L-tryptophan 2.1. Sodium bicarbonate was added to neutralize lysine-HCl.
^‡Methionine equivalents = g L-methionine + (g L-cyst(e)ine × 149/120).