Development of a Novel Cysteine Sulfinic Acid Decarboxylase Knockout Mouse: Dietary Taurine Reduces Neonatal Mortality

Figure 3

Schematic diagram for generation of different genotypes and sampling tissues for taurine concentrations, microarray analysis, and 𝑅𝑇² qPCR. Generations of different genotypes are shown as stepped horizontal lines. Short vertical lines indicate mating of 8-week-old mice. Short diagonal lines indicate birth of pups. Taurine treatment of G2 HO animals (double headed arrow) began 10 days before mating. Dashed vertical lines locate PD1. Asterisks (*) indicate observation of pup survival at PD1 and samples taken for taurine concentrations in the brain and liver. Hash marks (#) indicate observation of pup survival at PD1 and samples taken for taurine concentrations in the brain and/or liver as well as tissue samples taken at PD1 for microarray analysis and 𝑅𝑇² qPCR of the liver and brain mRNA.