Properties of Taurine Release in Glucose-Free Media in Hippocampal Slices from Developing and Adult Mice
Table 4
Effects of compounds involved in the second messenger systems on taurine release from mouse hippocampal slices in hypoxia.
Concentration (mM)
Efflux rate constants (×10−3 min−1) ± SEM
3-month-old
7-day-old
Basal (control)
2.36 ± 0.11
(20)
1.93 ± 0.18
(11)
1.34 ± 0.08
(31)
1.20 ± 0.11
(11)
+50 mM K+ (control)
2.92 ± 0.21
(10)
1.90 ± 0.10
(12)
Genistein 0.001
2.59 ± 0.13
(7)
2.06 ± 0.18
(7)
1.92 ± 0.16
(7)
1.44 ± 0.16
(4)
+50 mM K+
3.07 ± 0.25
(4)
2.27 ± 0.06
(4)
Quinacrine 0.01
1.20 ± 0.08
(7)
0.83 ± 0.05
(4)
0.75 ± 0.07
(8)
0.61 ± 0.06
(4)
+50 mM K+
1.12 ± 0.13
(4)
0.79 ± 0.05
(4)
PMA 0.00001
2.31 ± 0.20
(8)
1.77 ± 0.11
(4)
1.80 ± 0.15
(8)
1.33 ± 0.17
(4)
+50 mM K+
1.95 ± 0.17
(4)
1.85 ± 0.03
(4)
Chelerythrine 0.001
2.49 ± 0.17
(7)
2.07 ± 0.14
(4)
1.73 ± 0.18
(8)
1.53 ± 0.04
(4)
+50 mM K+
3.40 ± 0.51
(4)
2.01 ± 0.09
(4)
IBMX 1.0
1.85 ± 0.16
(8)
2.51 ± 0.29
(4)
1.99 ± 0.24
(6)
1.33 ± 0.11
(4)
+50 mM K+
2.70 ± 0.13
(4)
1.85 ± 0.03
(4)
RO 20-1724 0.2
3.46 ± 0.12
(8)
3.43 ± 0.15
(4)
1.96 ± 0.26
(7)
1.27 ± 0.17
(4)
+50 mM K+
4.56 ± 0.30
(4)
2.65 ± 0.33
(4)
Zaprinast 0.1
2.31 ± 0.15
(8)
2.00 ± 0.20
(8)
1.00 ± 0.13
(6)
0.66 ± 0.12
(4)
+50 mM K+
2.53 ± 0.06
(4)
2.04 ± 0.23
(4)
ODQ 0.01
2.51 ± 0.18
(7)
2.01 ± 0.13
(4)
1.50 ± 0.17
(8)
1.19 ± 0.08
(8)
+50 mM K+
2.50 ± 0.25
(4)
2.04 ± 0.06
(4)
Alloxan 5.0
2.81 ± 0.31
(7)
2.19 ± 0.06
(4)
1.70 ± 0.17
(6)
1.58 ± 0.25
(4)
+50 mM K+
3.13 ± 0.10
(4)
1.86 ± 0.07
(4)
The agonists were added at the beginning of the superfusion and 50 mM K+ at 30 min. The results show the efflux rate constants ± SEM (×10−3 min−1) for the time intervals of 20–30 min () and for 34–50 min without the excess of K+ or in the presence of 50 mM K+ () with the number of independent experiments in parenthesis. PMA: 4β-phorbol 12-myristate 13-acetate; IBMX: 3-isobutyl-1-methylxanthine; ODQ: 1H-1,2,4oxadiazolo4,3quinoxalin-1-one; RO 20-1724: 4-(3-butoxy-4-methoxyphenyl)-2-imidazolidone. Significance of differences from the corresponding controls: , .
