Genes of Different Catabolic Pathways Are Coordinately Regulated by Dal81 in Saccharomyces cerevisiae

Figure 1

Effect of Dal81 on gene induction. mRNA levels of UGA4 (a), DAL7 (b), AGP1 (c), and BAP2 (d) were determined in wild type cells (23344c strain), dal81Δ cells (SBCY17 strain), and dal81Δ cells transformed with the pSBC-HA-DAL81 plasmid. The second inducer, the specific one, was added 30 minutes after the addition of the first one and cells were incubated for another 30 minutes. mRNA levels were quantified by RT-qPCR. UGA4, DAL7, AGP1, and BAP2 values were normalized with TBP1 and results are the mean ± SEM of three independent experiments. Within each strain the values measured were normalized to the value obtained for the uninduced condition.