Genes of Different Catabolic Pathways Are Coordinately Regulated by Dal81 in Saccharomyces cerevisiae

Figure 2

Interaction of Dal81 with UGA4, AGP1, and DAL7 promoters. Wild type cells, expressing the HA-Dal81 (SBCY10 strain) fusion protein, were treated or not with the indicated inducers. ChIP assays were carried out using antibodies against the HA epitope. qPCR was performed with specific primers (black bars) that amplify a region of UGA4 promoter (a), a region of AGP1 promoter (b), a region of DAL7 promoter (c), and a region 2.5 kb downstream of UGA4 promoter used as a negative control (white bars). Results are expressed as the fold change of binding to the promoter of interest and are the mean ± SEM of three independent experiments.

(a)

(b)

(c)