Genes of Different Catabolic Pathways Are Coordinately Regulated by Dal81 in Saccharomyces cerevisiae

Table 2

Primers used in this work.

Primer group and name	Sequence (5′ to 3′)
Oligonucleotides for strain construction
F-leu3	TGCAATTATGGAAGGAAGATCAGATTTTGTGGCGACTTCACACAGCTGAAGCTTCGTACGC
R-leu3	GGACTTTAAACCTTGGGATTGAACGCAAATTCATTCATTAAACATAGGCCACTAAGTGGATCTG
F-STP1-HA	CAATATTTGAATTTTTACAATGACAACTTTGGGTCACAATTTCGGATCCCCGGGTTAATTAA
R-STP1-HA	TTCCAATATGATACCCTTATTTTTATCCCGTGTTATATTTAAGAATTCGAGCTCGTTTAAAC
F-LEU3 prom	AGGTGCCGCCTAATTTATCG
R-LEU3 int	ACTTCTGCTGACGACATTCC
F-KanMX6 int	CATCCTATGGAACTGCCTCG
R-KanMX6 int	GATAGATTGTCGCACCTGATTG
F-STP1 int	GCACAAGATAATCCTTCGTTCC
R-STP1 down	TCGGCTTTCCAATATGATACCC
R-Kan int	CTATACCTGAGAAAGCAACCTG
Oligonucleotides for RT-qPCR
F-qRT-UGA4	CTGCTGCTGTCACATTAACC
R-qRT-UGA4	AATACACATAACCACCACTGC
F-qRT-DAL7	AACCGAACAAATCAGGAAC
R-qRT-DAL7	CAAGTTGGAGATGAAGAGTC
F-qRT-BAP2	TAGAGGATGGCGTTGAGTC
R-qRT-BAP2	ACCAAGATGTAACCAATTATTAGC
F-qRT-AGP1	ATCTTATTCCTATTCTTGGCTACC
R-qRT-AGP1	CGGCGTTAATGAAGTGTGG
F-qRT-TBP1	TATAACCCCAAGCGTTTTGC
R-qRT-TBP1	GCCAGCTTTGAGTCATCCTC
Oligonucleotides for ChIP
F-UGA4	GGAACTGATTACTGTGCCAAG
R-UGA4	AATCGCTTATCGCTTATCGTG
F-AGP1	TTATACCTCGGCGGCTTC
R-AGP1	GCAAGATTTCTCCAAAGTCC
F-BAP2	AGGAGGCTACTGACACTGC
R-BAP2	GCTGACATATTTACCGTTGAAGG
F-DAL7	AATCTCCGCTGAAGTTGC
R-DAL7	TTTCACGATGTACCTTATCCAAGA
F-UC	AGTCCAATACCTCTGTCCTC
R-UC	AGCCGCAACTTCATTCTG