Urea Unfolding Study of E. coli Alanyl-tRNA Synthetase and Its Monomeric Variants Proves the Role of C-Terminal Domain in Stability

Figure 6

Near-UV CD spectra of WT-alaRS (a), G674D (b), N700 alaRS (c), and N461 alaRS (d) (15 μM) without denaturant (open circle), in the presence of 3 M urea (filled circle) and 8 M urea (open triangle). The buffer composition was the same as the far-UV CD and the temperature was kept at 25°C. The experiment was recorded with a cuvette having a path length of 10 mm and 50 nm/min scan speed was maintained. Five independent spectra were averaged.

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