Protein Quantification by Derivatization-Free High-Performance Liquid Chromatography of Aromatic Amino Acids

Figure 3

Chromatogram of an amino acid certified reference material (NIST 2389a) in 0.1 M HCl with added internal standards homotyrosine (HTyr) and 4-fluorophenylalanine (FPhe). In addition, a blank run is shown (dashed).