Protein Quantification by Derivatization-Free High-Performance Liquid Chromatography of Aromatic Amino Acids

Figure 5

Chromatogram of hydrolyzed bovine serum albumin (NIST SRM 927e, certified reference material) with added homotyrosine (HTyr) and 4-fluorophenylalanine (FPhe) used as internal standards. In addition, a blank line is shown (dashed).