Table of Contents
Journal of Amino Acids
Volume 2016 (2016), Article ID 7374316, 8 pages
http://dx.doi.org/10.1155/2016/7374316
Research Article

Protein Quantification by Derivatization-Free High-Performance Liquid Chromatography of Aromatic Amino Acids

Division 1.5 Protein Analysis, Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Strasse 11, 12489 Berlin, Germany

Received 15 April 2016; Accepted 28 June 2016

Academic Editor: Hieronim Jakubowski

Copyright © 2016 Almut Hesse and Michael G. Weller. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. L. Heinrikson and S. C. Meredith, “Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate,” Analytical Biochemistry, vol. 136, no. 1, pp. 65–74, 1984. View at Publisher · View at Google Scholar · View at Scopus
  2. R. A. Bank, E. J. Jansen, B. Beekman, and J. M. Te Koppele, “Amino acid analysis by reverse-phase high-performance liquid chromatography: improved derivatization and detection conditions with 9-fluorenylmethyl chloroformate,” Analytical Biochemistry, vol. 240, no. 2, pp. 167–176, 1996. View at Publisher · View at Google Scholar · View at Scopus
  3. S. Einarsson, B. Josefsson, and S. Lagerkvist, “Determination of amino acids with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography,” Journal of Chromatography A, vol. 282, pp. 609–618, 1983. View at Publisher · View at Google Scholar · View at Scopus
  4. P. Lindroth and K. Mopper, “High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde,” Analytical Chemistry, vol. 51, no. 11, pp. 1667–1674, 1979. View at Publisher · View at Google Scholar · View at Scopus
  5. S. A. Cohen and D. P. Michaud, “Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography,” Analytical Biochemistry, vol. 211, no. 2, pp. 279–287, 1993. View at Publisher · View at Google Scholar · View at Scopus
  6. D. H. Spackman, W. H. Stein, and S. Moore, “Automatic recording apparatus for use in the chromatography of amino acids,” Analytical Chemistry, vol. 30, no. 7, pp. 1190–1206, 1958. View at Publisher · View at Google Scholar · View at Scopus
  7. S. Moore, D. H. Spackman, and W. H. Stein, “Automatic recording apparatus for use in the chromatography of amino acids,” Federation Proceedings, vol. 17, no. 4, pp. 1107–1115, 1958. View at Google Scholar · View at Scopus
  8. S. Moore, D. H. Spackman, and W. H. Stein, “Chromatography of amino acids on sulfonated polystyrene resins—an improved system,” Analytical Chemistry, vol. 30, no. 7, pp. 1185–1190, 1958. View at Google Scholar
  9. P. Hušek, “Rapid derivatization and gas chromatographic determination of amino acids,” Journal of Chromatography A, vol. 552, no. 1-2, pp. 289–299, 1991. View at Publisher · View at Google Scholar · View at Scopus
  10. A. G. Calder, K. E. Garden, S. E. Anderson, and G. E. Lobley, “Quantitation of blood and plasma amino acids using isotope dilution electron impact gas chromatography/mass spectrometry with U-13C amino acids as internal standards,” Rapid Communications in Mass Spectrometry, vol. 13, no. 21, pp. 2080–2083, 1999. View at Publisher · View at Google Scholar · View at Scopus
  11. C. Meinert and U. J. Meierhenrich, “Derivatization and multidimensional gas-chromatographic resolution of α-alkyl and α-dialkyl amino acid enantiomers,” ChemPlusChem, vol. 79, no. 6, pp. 781–785, 2014. View at Publisher · View at Google Scholar · View at Scopus
  12. I. Myrgorodska, C. Meinert, Z. Martins, L. Le Sergeant D'Hendecourt, and U. J. Meierhenrich, “Molecular chirality in meteorites and interstellar ices, and the chirality experiment on board the ESA cometary rosetta mission,” Angewandte Chemie—International Edition, vol. 54, no. 5, pp. 1402–1412, 2015. View at Publisher · View at Google Scholar · View at Scopus
  13. Y.-F. Cheng and N. J. Dovichi, “Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence,” Science, vol. 242, no. 4878, pp. 562–564, 1988. View at Publisher · View at Google Scholar · View at Scopus
  14. N. V. Gogichaeva and M. A. Alterman, “Amino acid analysis by means of MALDI TOF mass spectrometry or MALDI TOF/TOF tandem mass spectrometry,” Methods in Molecular Biology, vol. 828, pp. 121–135, 2012. View at Publisher · View at Google Scholar · View at Scopus
  15. J. Qu, Y. Wang, G. Luo, Z. Wu, and C. Yang, “Validated quantitation of underivatized amino acids in human blood samples by volatile ion-pair reversed-phase liquid chromatography coupled to isotope dilution tandem mass spectrometry,” Analytical Chemistry, vol. 74, no. 9, pp. 2034–2040, 2002. View at Publisher · View at Google Scholar · View at Scopus
  16. T. Konz, M. Montes-Bayón, J. Bettmer, and A. Sanz-Medel, “Analysis of hepcidin, a key peptide for Fe homeostasis, via sulfur detection by capillary liquid chromatography-inductively coupled plasma mass spectrometry,” Journal of Analytical Atomic Spectrometry, vol. 26, no. 2, pp. 334–340, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. E. Rampler, T. Dalik, G. Stingeder, S. Hann, and G. Koellensperger, “Sulfur containing amino acids—challenge of accurate quantification,” Journal of Analytical Atomic Spectrometry, vol. 27, no. 6, pp. 1018–1023, 2012. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Rappel and D. Schaumlöffel, “The role of sulfur and sulfur isotope dilution analysis in quantitative protein analysis,” Analytical and Bioanalytical Chemistry, vol. 390, no. 2, pp. 605–615, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. Y. Suzuki, A. Nobusawa, and N. Furuta, “Quantification of proteins by measuring the sulfur content of their constituent peptides by means of nano HPLC-ICPMS,” Analytical Sciences, vol. 30, no. 5, pp. 551–559, 2014. View at Publisher · View at Google Scholar · View at Scopus
  20. N. Zinn, R. Krüger, P. Leonhard, and J. Bettmer, “μLC coupled to ICP-SFMS with post-column isotope dilution analysis of sulfur for absolute protein quantification,” Analytical and Bioanalytical Chemistry, vol. 391, no. 2, pp. 537–543, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. R. I. Krohn, “The colorimetric detection and quantitation of total protein,” in Handbook of Food Analytical Chemistry, R. E. Wrolstad, Ed., pp. 77–104, John Wiley & Sons, Hoboken, NJ, USA, 2000. View at Google Scholar
  22. P. K. Smith, R. I. Krohn, G. T. Hermanson et al., “Measurement of protein using bicinchoninic acid,” Analytical Biochemistry, vol. 150, no. 1, pp. 76–85, 1985. View at Publisher · View at Google Scholar · View at Scopus
  23. M. H. Simonian, “Spectrophotometric determination of protein concentration,” in Handbook of Food Analytical Chemistry, R. E. Wrolstad, Ed., pp. 115–121, John Wiley & Sons, New Jersey, NJ, USA, 2000. View at Google Scholar
  24. K. C. Rhee, “Determination of total nitrogen,” in Handbook of Food Analytical Chemistry, R. E. Wrolstad, Ed., pp. 105–113, John Wiley & Sons, Hoboken, NJ, USA, 2000. View at Google Scholar
  25. J. Kjeldahl, “Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern,” Zeitschrift für Analytische Chemie, vol. 22, no. 1, pp. 366–382, 1883. View at Publisher · View at Google Scholar · View at Scopus
  26. Questions and Answers on melamine, 2008, http://www.who.int/csr/media/faq/QAmelamine/en/.
  27. K. R. Allen, T. J. Degg, P. A. Rushworth, M. Smith, and M. J. Henderson, “Measurement of phenylalanine and tyrosine in plasma by high-performance liquid chromatography using the inherent fluorescence of aromatic amino acids,” Annals of Clinical Biochemistry, vol. 36, no. 2, pp. 207–211, 1999. View at Publisher · View at Google Scholar · View at Scopus
  28. I. Molnar-Perl, Quantitation of Amino Acids and Amines by Chromatography—Methods and Protocols, 2005.
  29. M. Fountoulakis and H.-W. Lahm, “Hydrolysis and amino acid composition analysis of proteins,” Journal of Chromatography A, vol. 826, no. 2, pp. 109–134, 1998. View at Publisher · View at Google Scholar · View at Scopus
  30. Y. Li, A.-G. Tang, and S. Mu, “HPLC-FLD determination of serum aromatic amino acids: application in chronic kidney disease patients,” Clinica Chimica Acta, vol. 412, no. 11-12, pp. 1032–1035, 2011. View at Publisher · View at Google Scholar · View at Scopus
  31. Y. Dale, V. Mackey, R. Mushi, A. Nyanda, M. Maleque, and J. Ike, “Simultaneous measurement of phenylalanine and tyrosine in phenylketonuric plasma and dried blood by high-performance liquid chromatography,” Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 788, no. 1, pp. 1–8, 2003. View at Publisher · View at Google Scholar · View at Scopus
  32. G. Winkler, P. Wolschann, P. Briza et al., “Spectral properties of trifluoroacetic acid acetonitrile gradient systems for separation of picomole quantities of peptides by reversed phase high performance liquid chromatography,” Journal of Chromatography, vol. 347, no. 1, pp. 83–88, 1985. View at Publisher · View at Google Scholar