Table of Contents
Journal of Applied Chemistry
Volume 2018, Article ID 7253639, 6 pages
Research Article

Study on the Factors Influencing the Extraction of Chenodeoxycholic Acid from Duck Bile Paste by Calcium Salt Method

College of Food Engineering & Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China

Correspondence should be addressed to Xiangzheng Hu; nc.ude.tsut@hzxuh

Received 26 June 2017; Revised 5 December 2017; Accepted 25 December 2017; Published 1 March 2018

Academic Editor: Alberto Figoli

Copyright © 2018 Xiangzheng Hu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


New extraction technology of chenodeoxycholic acid from duck bile paste by calcium salt was investigated. The optimum conditions of extraction were determined by orthogonal experimental design. The results indicated that influencing factors on the extraction efficiency of chenodeoxycholic acid were as follows: hydrogen peroxide, methyl alcohol, glacial acetic acid, and calcium chloride. The optimum extracting conditions of chenodeoxycholic acid were 1000 mL amount of methyl alcohol, 50 mL amount of hydrogen peroxide, 500 mL amount of 20% calcium chloride, and 600 mL amount of 60% glacial acetic acid for a quantity of duck paste. The yield of chenodeoxycholic acid was 30%.