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Journal of Automatic Chemistry
Volume 14, Issue 4, Pages 137-143

Application and automation of flow injection analysis (FIA) using fast responding enzyme glass electrodes to detect penicillin in fermentation broth and urea in human serum

Laboratoire de Biotecknologie, Ecole des Mines de Saint-Etienne, Saint- Etienne Cedex 02 42023, France

Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An enzyme immobilization technique has been developed to determine the concentration of biological compounds. This technique has been applied to penicillinase and urease, which are crosslinked as very fine films directly onto the sensitive ends of pH glass electrodes, thereby dispensing with the need of an on-line enzyme reactor. The biosensor is incorporated in an FIA system within a magnetically stirred detection cell. Penicillin-V in fermentation broth and urea in human serum samples were detected and the results were compared with HPLC and spectrophotometric methods. On-line measurement is achieved through the automation of this FIA system.