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Journal of Automatic Chemistry
Volume 20, Issue 6, Pages 195-198
http://dx.doi.org/10.1155/S146392469800025X

Diagnosis of infections in newborns using a new particle-mediated immunoassay for serum C-reactive protein

1Department of Clinical and Laboratory Medicine, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno, Osaka 545, Japan
2Department of Clinical Hematology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno, Osaka 545, Japan
3Department of Pediatrics, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno, Osaka 545, Japan
4Department of Obstetrics and Gynecology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno, Osaka 545, Japan

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

C-reactive protein (CRP) levels were measured using a new particle-mediated immunoassay. Tests for precision and linearity of this method gave satisfactory results. The minimum sensitivity of the assay was 1 ng/ml. Interference by bilirubin (<220mg/l) and haemoglobin (<20g/l) was not observed. Using this method, CRP was assayed as a means of monitoring for infection in newborns up to 72 h after delivery. The pattern of time course elevation curves was similar for both groups (10 healthy subjects and 26 patients), but the serum CRP (ng/ml) of infected newborns rose significantly higher than in healthy subjects at 24 h after birth. The rate of increase of CRP (∆CRP; ng/ml/h) may be a more useful parameter to detect infection, since a significant change in ∆CRP was apparent only 12 h after birth. The reported method was reliable and the parameters obtained were considered clinically useful for early detection of infection.