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Journal of Automated Methods and Management in Chemistry
Volume 2007, Article ID 86857, 7 pages
http://dx.doi.org/10.1155/2007/86857
Research Article

The Application of Resonance Light Scattering Technique for the Determination of Tinidazole in Drugs

1College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
2Hunan Wantwant Hospital, Changsha 410016, China
3Research Institute for Molecular Pharmacology and Therapeutics, Central South University, Changsha 410083, China
4Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York 10021, NY, USA

Received 13 January 2007; Accepted 26 April 2007

Copyright © 2007 Xin Yu Jiang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A resonance light scattering technique to determine tinidazole in drugs was developed by tetraphenylboron sodium (TPB). Tinidazole was found to bind B(C6H5)4 anion and transformed to tinidazole-TPB aggregate which displayed intense resonance scattering light. Effects of factors such as wavelength, acidity, stabilizers, and interferents on the RLS of tinidazole TPB were investigated in detail. The RLS intensity of the tinidazole-TPB suspension was obtained in sulfuric acid solution (pH=1.44). The resonance scattering light intensity at the maximum RLS peak of 569.5 nm was linear to the concentration of tinidazole in the range of 10.0–30.0 μgmL1 with a detection limit of 5.0 μgmL1. Good results were also obtained with the recovery range of 95.13–106.76%. The method was applied to determine tinidazole in injections and tablets, showing high sensitivity and accuracy compared with the high performance liquid chromatography method (HPLC) according to Chinese Pharmacopoeia.