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Journal of Automated Methods and Management in Chemistry
Volume 2008, Article ID 937651, 8 pages
Research Article

Ascorbic Acid Determination in Commercial Fruit Juice Samples by Cyclic Voltammetry

1Chemistry and Biochemistry Department, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine-Bucharest, Bulevardul Marasti, no 59, 011464 Bucharest, Romania
2Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, Panduri Avenue 90-92, 050657 Bucharest, Romania
3Department of Chemistry, University of Warmia and Mazury in Olsztyn, 10-975 Olsztyn, Poland

Received 12 December 2008; Accepted 23 December 2008

Academic Editor: Peter Stockwell

Copyright © 2008 Aurelia Magdalena Pisoschi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A method was developed for assessing ascorbic acid concentration in commercial fruit juice by cyclic voltammetry. The anodic oxidation peak for ascorbic acid occurs at about 490 mV on a Pt disc working electrode (versus SCE). The influence of the potential sweep speed on the peak height was studied. The obtained calibration graph shows a linear dependence between peak height and ascorbic acid concentration in the domain (0.1–10 mmolL1). The equation of the calibration graph was 𝑦=6.391𝑥+0.1903 (where 𝑦 represents the value of intensity measured for the anodic peak height, expressed as 𝜇A and 𝑥 the analyte concentration, as mmolL1, 𝑟2=0.9995, r.s.d.=1.14%, 𝑛=10, Cascorbicacid=2 mmolL1). The developed method was applied to ascorbic acid assessment in fruit juice. The ascorbic acid content determined ranged from 0.83 to 1.67 mmolL1 for orange juice, from 0.58 to 1.93 mmolL1 for lemon juice, and from 0.46 to 1.84 mmolL1 for grapefruit juice. Different ascorbic acid concentrations (from standard solutions) were added to the analysed samples, the degree of recovery being comprised between 94.35% and 104%. Ascorbic acid determination results obtained by cyclic voltammetry were compared with those obtained by the volumetric method with dichlorophenol indophenol. The results obtained by the two methods were in good agreement.