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Journal of Analytical Methods in Chemistry
Volume 2012, Article ID 101249, 8 pages
Research Article

Bioanalytical Method Development and Validation of Memantine in Human Plasma by High Performance Liquid Chromatography with Tandem Mass Spectrometry: Application to Bioequivalence Study

1Department of Pharmaceutical Chemistry, Hindu College of Pharmacy, Amaravathi Road, Guntur, Andhrapradesh 522002, India
2Department of Chemistry, Jawaharlal Nehru Technological University, Anantapur 515002, India
3Department of Pharmaceutical Analysis, Nirmala College of Pharmacy, Madras Road, Kadapa, Andhrapradesh 516002, India
4Department of Pharmaceutical Sciences, Donbosco College of Pharmacy, Pulladigunta, Guntur 522201, India

Received 3 November 2011; Revised 3 January 2012; Accepted 9 January 2012

Academic Editor: Antonio Ruiz-Medina

Copyright © 2012 Ravi Kumar Konda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple, sensitive, and rapid HPLC-MS/MS method was developed and validated for quantitative estimation of memantine in human plasma. Chromatography was performed on Zorbax SB-C18 ( 4 . 6 × 7 5  mm, 3.5 μm) column. Memantine (ME) and internal standard Memantine-d6(MED6) were extracted by using liquid-liquid extraction and analyzed by LC-ESI-MS/MS using multiple-reaction monitoring (MRM) mode. The assay exhibited a linear dynamic range of 50.00–50000.00 pg/ml for ME in human plasma. This method demonstrated an intra- and interday precision within the range of 2.1–3.7 and 1.4–7.8%, respectively. Further intra- and interday accuracy was within the range of 95.6–99.8 and 95.7–99.1% correspondingly. The mean recovery of ME and MED6 was 8 6 . 0 7 ± 6 . 8 7 and 8 0 . 3 1 ± 5 . 7 0 %, respectively. The described method was successfully employed in bioequivalence study of ME in Indian male healthy human volunteers under fasting conditions.