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Journal of Analytical Methods in Chemistry
Volume 2012, Article ID 810156, 12 pages
http://dx.doi.org/10.1155/2012/810156
Research Article

Sensitive Spectrophotometric Determination of Atenolol in Pharmaceutical Formulations Using Bromate-Bromide Mixture as an Eco-Friendly Brominating Agent

Department of Chemistry, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India

Received 25 November 2011; Accepted 27 December 2011

Academic Editor: Dario Compagnone

Copyright © 2012 Kudige N. Prashanth and Kanakapura Basavaiah. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Three simple and sensitive spectrophotometric methods are proposed for the determination of atenolol (ATN) in bulk drug and tablets. The methods are based on the bromination of ATN by the bromine generated in situ by the action of the acid on the bromate–bromide mixture followed by the determination of unreacted bromine by reacting with a fixed amount of either meta-cresol purple (MCP) and measuring the absorbance at 540 nm (method A) and 445 nm (method B) or erioglaucine (EGC) and measuring the absorbance at 630 nm (method C). Beer's law is valid within the concentration ranges of 1.0–20.0, 2.0–40.0 and 1.0–8.0 μg/mL for method A, method B and method C, respectively. The calculated molar absorptivities were found to be 1.20×104, 4.51×103 and 3.46×104  L/molcm for method A, method B and method C, respectively. Sandell’s sensitivity values, correlation coefficients, limits of detection and quantification are also reported. Recovery results were statistically compared with those of a reference method by applying Student’s t- and F-test. The novelty of the present study is the measurement of two different colors using MCP, that is, red-pink color of MCP in acid medium at 540 nm and yellowish-orange color of brominated MCP at 445 nm.