A Validated Reverse Phase HPLC Analytical Method for Quantitation of Glycoalkaloids in Solanum lycocarpum and Its Extracts

Table 2

Linear regression to quantify glycoalkaloids.

Compounds	Linear range	Linear coefficient (a)	Angular coefficient (b)	Correlation coefficient (r)
SN	0.77–990.00	5603.2	9026.0	0.9996
SM	0.78–1000.00	6433.3	9204.0	0.9996
IS	1.62–12.50	97525.0	1227.3	0.9998
SS	24.75–990.00	7996.1	8856.7	0.9992

Analysis with a regression equation of y = ax + b, in which 𝑥 is the concentration in μg mL⁻¹, y is the peak area, a is angular coefficient, and 𝑏 is linear coefficient. SN: solasonine, SM: solamargine, IS: internal standard (veratraldehyde), and SS: secondary standard (solanine).