Combination of ERG9 Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene in Saccharomyces cerevisiae

Figure 5

SDS/PAGE of recombinant enzymes produced in different yeast strains. Lane M: molecular mass standards; lane a, crude extract FPPS; lane b: purified FPPS; lane c: crude extract ADS; lane d: purified ADS; lane e: crude extract FPPS-ADS; lane f: purified FPPS-ADS lane g: crude extract ADS-FPPS; lane h: purified ADS-FPPS. The calculated molecular weights of FPPS, ADS, and the fusion enzymes were 40, 63, and 103 kDa, respectively.