Research Article

Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles

Table 1

Experimental design matrix for the robustness study and response values ( ).

PatternAcetonitrile percent in water (%) v/vWavelength (nm)pHFlow rate (mL·min−1)

322283.71.1
322323.70.9
282323.90.9
322323.91.1
282323.71.1
282283.70.9
282283.91.1
322283.90.9
Low level 28 228 3.7 0.9
High level 32 232 3.9 1.1