Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum

Table 4

Robustness of the method.

Chromatographic conditions	Variation	Retention time (minutes)
		PG	TMP
Temperature (°C)	23	3.099	6.346
	27	3.217	6.381
Flow rate (mL/min.)	0.9	3.341	6.413
	1.1	2.89	5.994
Vol. of acetonitrile (%)	48	3.397	6.499
	52	2.982	5.619