Research Article
Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum
Table 5
Summary of forced degradation results.
| | Stress conditions | Time (h) | Assay of active substance (%) | Increase due to oxidation (%) | | PG (100 µg/mL) | TMP (0.1 µg/mL) | PG (100 µg/mL) | TMP (0.1 µg/mL) |
| Acid hydrolysis
(0.1 M HCl) | 1 | 92.88 | 96.46 | 7.12 | 3.54 | Base hydrolysis
(0.5 M NaOH) | 1 | 129.17 | 101.09 | 29.17 | 1.09 | Oxidation
(30% H2O2) | 1 | 623.16 | 103.14 | 523.16 | 3.14 | | Thermal (60°C) | 1 | 106.53 | 105.94 | 6.53 | 5.94 |
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