Journal of Analytical Methods in Chemistry / 2013 / Article / Fig 3

Research Article

Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical Neurons

Figure 3

AKT signaling pathway is required for the neuroprotective effect of CE. (a) representative western blot probed with an antibody specific against p-AKT after various treatments in the neurons. Actin served as an internal control. Summary of the optical density of p-AKT. Results showed that CE significantly inhibited H2O2 induced decreases in the content of p-AKT. * refers to a comparison of the control group; # refers to a comparison of the H2O2 group. (b) Bar graphs showing that the neuroprotective effect of CE was blocked by AKT inhibitor Ly294002, suggesting that AKT signaling pathway mediated the neuroprotective effect of CE in primary cultured cortical neurons.

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