Rapid Screening of Drug-Protein Binding Using High-Performance Affinity Chromatography with Columns Containing Immobilized Human Serum Albumin

Table 1

The retention time, reported PPB, and predicted pKa of reference compounds.

Compound	Retention time; (min)	PPB from; literature (%)	pKa based on; ACD/labs
Caffeic acid	4.02	66.0	4.04 ± 0.40
Caffeine	1.18	22.5	0.63 ± 0.70
Catechuic acid	1.94	20.7	4.45 ± 0.10
Chlorphenamine maleate	5.93	37.5 (25–50)	3.77 ± 0.19
Chuanxiongzine hydrochloride	2.23	44.3	2.88 ± 0.50
Diazepam	132.21	94.5 (90–99)	3.40 ± 0.10
Ferulic acid	1.79	20.6	4.04 ± 0.40
Fluconazole	1.07	11.5 (11-12)	2.56 ± 0.12
Ranitidine hydrochloride	1.23	15.0 (12–18)	2.38 ± 0.70
Phenytoin sodium	15.02	90.0	0.21 ± 0.40
Praziquantel	12.97	80.0	−0.79 ± 0.20
Salicylic acid	21.81	85.0 (80–90)	3.01 ± 0.10
Sulfadiazine	1.88	43.0 (38–48)	1.57 ± 0.10
Sulfadimidine	6.22	80.0	1.55 ± 0.10
Sulfamethoxazole	3.68	69.5 (69-70)	1.39 ± 0.10
Tolbutamide	35.82	90.0	5.12 ± 0.50
Vanillic acid	3.11	56.3	4.45 ± 0.10
Warfarin	87.85	98.0	4.50 ± 1.00
Bendroflumethiazide	3.27	94.0	8.63 ± 0.40
Diphenhydramine hydrochloride	6.23	98.0	8.76 ± 0.28
Hydrocortisone acetate	0.73	90.0	13.46 ± 0.70
Rutoside	13.43	85.0 (80–90)	13.85 ± 0.70
Nefopam hydrochloride	7.87	73.5 (71–76)	9.16 ± 0.70
Phenacetin	3.19	30.0	14.57 ± 0.70
Progesterone	50.02	97.0	19.28*
Propranolol hydrochloride	5.21	92.5 (90–95)	9.15 ± 0.38
Quercetin	0.72	98.0	8.14 ± 0.60
Reserpine	0.71	96.0	7.25 ± 0.70
Testosterone propionate	0.74	98.0	9.63 ± 0.65

*Calculated by software of Pallas.