Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats
Figure 3
Representative MS, MS/MS spectrum, and extracted ion chromatogram (EIC) of in vitro metabolites of BG in rat liver microsomes (RLM). Incubations were performed at 37°C for 30 min in a system containing 3.06 mg/L BG and 0.5 mg/mL liver microsomal protein in 0.5 mL of 0.1 mol/L phosphate buffer (pH 7.4). (a) MS spectrum; (b) MS/MS spectrum and the predominant fragmentation pattern of M1a are shown as the representative spectra of five metabolites, which exhibited almost identical spectra; (c) EIC of m/z 321.1339 representing 5 isomers of in vitro metabolites with their RTs at 49.0, 51.8, 54.0, 56.8, and 57.5 min, respectively.
