Cloud-Point Extraction Combined with Liquid Chromatography for the Determination of Ergosterol, a Natural Product with Diuretic Activity, in Rat Plasma, Urine, and Faeces

Table 1

Summary of precision and accuracy of ergosterol in rat plasma, urine, and faeces.

Added concentration; (𝜇g/mL)	Found concentration; (mean ± SD, 𝜇g/mL)	Recovery (%)	Intraday (RSD %)	Interday (RSD %)
Plasma
0.25	0.259±0.012	103.6	9.6	7.8
0.75	0.713±0.030	95.1	3.7	5.4
2.00	2.001±0.072	100.1	3.9	4.8
Urine
0.25	0.254±0.009	101.6	4.2	7.1
0.75	0.736±0.038	98.1	5.5	5.8
2.00	1.956±0.090	97.8	3.3	3.1
Faeces
0.25	0.247±0.010	98.8	4.8	3.6
25	25.1±0.7	100.4	2.8	5.1
100	99.5±1.9	99.5	3.1	1.6