LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

Table 3

Composition of the fraction of conjugated phytosterols in the sea buckthorn leaves determined by HPLC-NMR.

Compound	Comparative content^a	Overall content in the leave extract^b	Retention time (min)^c
𝛼-amyrin + FA	11%	0.6%	15
𝛽-sitosterol + unsat. FA	18%	1.0%	20
𝛽-sitosterol + sat. FA	12%	0.6%	22
𝛽-smyrin + unsat. FA	39%	2.1%	25
𝛽-amyrin + sat. FA	20%	1.1%	26

aMolar ratio in the phytosterol fraction, ^bweight ratio in the extract sample, and ^cisocratic method (CDCl₃ : acetonitrile, 50 : 50).