Research Article

Optimization of IC Separation Based on Isocratic-to-Gradient Retention Modeling in Combination with Sequential Searching or Evolutionary Algorithm

Figure 5

Comparison of experimental and predicted chromatograms obtained with optimal gradient profile: (a) predicted optimal separation by simplex algorithm, (b) predicted optimal separation by genetic algorithm, (c) real chromatogram corresponding to simplex optimization, and (d) real chromatogram corresponding to genetic algorithm. Dashed lines indicate the calculated optimal gradients. Analyzed sugars are marked according to elution order: arabitol, fucose, N-acetyl-D-glucosamine, fructose, melibiose, lactulose, raffinose, and cellobiose.