Several Affinity Tags Commonly Used in Chromatographic Purification
Figure 1
(a) Schematic illustration of the N-terminal tagged fusion protein. The spacer represents an endopeptidase cleavage sequence and/or solubility and folding enhancers. (b) Principle of fusion protein affinity purification and removal of the tag (only for N-terminal tagging). The interaction proteins will be copurified with the tagged fusion protein under native conditions.
