Fluorimetric Immunoassay for Multianalysis of Aflatoxins

Table 1

Reported biosensing techniques for analysis of AFM1 and AFB1.

Biosensing technique	Analyte	Detection limit	Reference
Surface plasmon resonance (SPR) immunoassay	AFB1	3 ng/mL	[13]
Optical (chemiluminescent) enzyme immunoassay	AFB1	0.01 ng/g	[14]
Long range SPR	AFM1	0.6 pg/mL	[15]
SPR	AFB1	0.2 ng/mL	[8]
Impedance	AFM1	15 ng/L	[16]
Impedance and cyclic voltammetry	AFM1	1 ng/mL	[17]
Bioelectronic recognition assay	AFM1, AFB1	5 pg/mL	[18]
Linear sweep voltammetry	AFM1	0.054 ng/mL	[6]
Direct competitive ELISA (MNPs)	AFM1	8 ng/L	[19]
Indirect competitive ELISA	AFM1	5 ng/L	[20]
Indirect competitive ELISA	AFM1	0.24 ng/mL	[21]
Flow-injection immunoassay	AFM1	11 pg/mL	[22]
Competitive ELISA	AFM1	28 ng/kg	[23]
Indirect competitive ELISA	AFM1	0.04 ng/mL	[24]
Competitive ELISA	AFM1	39 ng/L	[25]
SPR	AFB1, AFG1	0.97 g/kg	[26]
Chemiluminescent ELISA	AFM1	0.05 pg/mL	[27]
Electrochemical impedance spectroscopy	AFM1	1 pg/mL	[28]
Fluorometric ELISA	AFM1, AFM2,; AFB1, AFG1	1 pg/mL	This work