UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit

<table>The typical total ion chromatogram (TIC) of C. mas fruit puree. Ionization source performed on Agilent Jet Stream TOF-MS with electrospray ionization (ESI) negative mode. (1) x-axis represents retention time and y-axis is intensity of <svg height="14.85" id="M8" style="vertical-align:-2.22495pt" version="1.1" viewbox="0 0 29 14.85" width="29" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,12.012)"><path d="M766 88q-40 -45 -83.5 -72.5t-63.5 -27.5q-39 0 -16 103l49 224q15 68 -10 68q-36 0 -112.5 -78.5t-105.5 -133.5q-27 -101 -38 -165q-35 -4 -77 -18l-6 6q42 153 70 288q12 55 10 78t-19 23q-38 0 -114 -80.5t-101 -131.5q-24 -68 -41 -165q-36 -3 -76 -18l-8 6
q56 202 87 347q7 33 -3 33q-8 0 -31.5 -17.5t-41.5 -35.5l-12 28q42 45 84 72t63 27q44 0 10 -124l-20 -75h2q92 121 193 178q36 21 64 21q62 0 28 -159l-8 -37h2q50 67 103.5 112t94.5 65q39 19 62 19q60 0 23 -156l-45 -189q-9 -39 2 -39q17 0 71 49z" id="x1D45A"></path></g><g transform="matrix(.017,-0,0,-.017,13.475,12.012)"><path d="M368 703l-264 -866h-60l265 866h59z" id="x2F"></path></g><g transform="matrix(.017,-0,0,-.017,20.478,12.012)"><path d="M475 445l-64 -69q-119 -128 -289 -280q27 6 81 -15q66 -26 102 -26q48 0 111 93l23 -19q-41 -84 -75.5 -116.5t-73.5 -32.5q-37 0 -147 53q-43 21 -63 7q-22 -15 -38 -41q-4 -7 -11 -7q-8 10 -8 26q0 38 47 83l292 273q-32 -5 -67 -1q-63 7 -105 7q-32 0 -53.5 -22
t-47.5 -67l-25 13q15 39 38 78q40 66 83 66q57 0 120 -12q52 -10 80 -10q29 0 67 42z" id="x1D467"></path></g>
</svg> peaks. (2) Peaks 1–10 were identified as tartaric acid (1), malic acid (2), citric acid (3), gallic acid (4), chlorogenic acid (6), rutin (9), and four iridoids, loganic acid (5), loganin (7), sweroside (8), and cornuside (10). (3) Peaks 6, 7, and 9 were enlarged by zooming in.</table>

Journal of Analytical Methods in Chemistry

fig1

Figure 1

Figure 1: UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit