Reliable HPLC Determination of Aflatoxin M1 in Eggs

Table 1

Trueness calculations and recoveries (mean ± SD, 𝑛=6) for 3 spiking levels in eggs, LOD, LOQ, CRM¹, and PT² for AFM1.

Mycotoxin	Commodity	Spiking level (µg/kg)	Recovery (%)	𝑋−𝑇^∗	Bias (RD %)^#	LOD (µg/kg)	LOQ (µg/kg)	FAPAS CRM T04120 Accepted range (0.33–0.84) µg/kg	FAPAS PT T04124 Assign value (0.505 µg/kg)
AFM1		0.05	95.3±9.3	2.3	4.7				0.29, 𝑍-score = −1.9
	Eggs	0.10	98.0±6.0	2.0	2.0	0.012	0.05	0.64
		0.50	87.1±6.7	64.3	12.9

1Certified reference materials.
2Proficiency test.
^∗𝑋: expected value, 𝑇: mean value.
#Relative difference.