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Journal of Analytical Methods in Chemistry
Volume 2013, Article ID 932946, 8 pages
Research Article

Portable Bioactive Paper-Based Sensor for Quantification of Pesticides

1NanoBiz Ltd. Metu Technopolis, Galyum Block, Floor 2, No. 18, 06800 Ankara, Turkey
2Department of Biology, Nanobiotechnology R&D Lab, Middle East Technical University, 06800 Ankara, Turkey
3School of Medicine, Istanbul Kemerburgaz University, 34217 Istanbul, Turkey

Received 14 May 2013; Revised 13 June 2013; Accepted 1 July 2013

Academic Editor: Miren Lopez de Alda

Copyright © 2013 Murat Kavruk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A paper-based biosensor was developed for the detection of the degradation products of organophosphorus pesticides. The biosensor quantifies acetylcholine esterase inhibitors in a fast, disposable, cheap, and accurate format. We specifically focused on the use of sugar or protein stabilizer to achieve a biosensor with long shelf-life. The new biosensor detected malathion with a detection limit of 2.5 ppm in 5 min incubation time. The operational stability was confirmed by testing 60 days storage at 4°C when glucose was used as stabilizer.