Simultaneous Determination of Reserpine, Rescinnamine, and Yohimbine in Human Plasma by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry

Table 1

Intra- and inter-day precision and accuracy of reserpine, rescinnamine and yohimbine in plasma.

Analyte	Nominal concentration (ng/mL)	Intraday variation (𝑛=6)			Inter-day variation (𝑛=18)
		Measured conc. (ng/mL ± SD)	Precision (CV, %)	Accuracy (recovery, %)	Measured conc. (ng/mL ± SD)	Precision (CV, %)	Accuracy (recovery, %)
Reserpine	0.96	1.08±0.04	3.7	112.5	1.07±0.06	6.0	111.1
	24	23.9±1.99	8.3	99.7	22.9±1.76	7.6	95.6
	300	269.3±13.4	4.9	89.8	272.4±17.6	6.4	111.8
Rescinnamine	0.80	0.87±0.04	5.0	108.1	0.86±0.05	5.4	108.1
	20	19.3±1.86	9.6	96.7	20.54±1.91	9.3	102.7
	250	228.0±9.9	4.3	91.2	238.0±16.78	7.0	95.2
Yohimbine	0.64	0.71±0.04	5.4	110.4	0.69±1.03	4.6	108.1
	16	17.0±1.67	9.8	106.3	17.06±1.43	8.4	106.6
	200	182.2±5.1	2.7	91.1	183.39±6.71	3.6	91.2