Journal of Analytical Methods in Chemistry

Journal of Analytical Methods in Chemistry / 2013 / Article
Special Issue

Chemistry of Medicinal Plants, Foods, and Natural Products

View this Special Issue

Research Article | Open Access

Volume 2013 |Article ID 949830 |

Xue Meng, Xinfeng Zhao, Shixiang Wang, Pu Jia, Yajun Bai, Sha Liao, Xiaohui Zheng, "Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study", Journal of Analytical Methods in Chemistry, vol. 2013, Article ID 949830, 7 pages, 2013.

Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study

Academic Editor: Shixin Deng
Received20 Jul 2013
Accepted15 Oct 2013
Published17 Nov 2013


A sensitive and specific gas chromatographic-mass spectrometry with selected ion monitoring (GC-MS/SIM) method has been developed for simultaneous identification and quantification of α-asarone, -asarone, and methyl eugenol of Acorus tatarinowii Schott in rat plasma. Chromatographic separation was performed on a Restek Rxi-5MS capillary column (30 m × 0.32 mm × 0.25  m), using 1-naphthol as internal standard (IS). MS detection of these compounds and IS was performed at m/z 178, 208, 208, and 144. Intra- and interday precisions of all compounds of interest were less than 10%. The recoveries are situated in the range of 92.4–105.2%. Pharmacokinetics of methyl eugenol confirmed to be one-compartment open model, α-asarone and β-asarone was two-compartment open model, respectively. The method will probably be an alternative to simultaneous determination and pharmacokinetic study of volatile ingredients in Acorus tatarinowii Schott.

1. Introduction

Chinese medicines have played an important role in clinical therapy in China, Korea, and Japan attributed to high pharmacological activity and few complications. As a classic medicinal plant, the dry rhizome of Acorus tatarinowii Schott is officially listed in the Chinese Pharmacopoeia and widely used for fighting ailments such as epilepsy, digestive disorders, cerebrovascular disease, stroke, senile dementia, and diarrhea [15]. Recent studies have found that aqueous extract and volatile oil of Acorus tatarinowii Schott dose-dependently suppressed the intensity of APO-induced stereotypic behavior and locomotor activity and extended the duration of sleeping induced by pentobarbital [6, 7]. Inhalation of the volatile oil also has the properties of reversing decreased brain GABA levels induced by PTZ inhibition of GABA transaminase [8].

Low polar or nonpolar compounds including -asarone, -asarone, and methyl eugenol proved to be main bioactive ingredients in volatile oil of Acorus tatarinowii Schott in previous report [9]. -Asarone has significant pharmacological effects on cardiovascular and central nervous system, while -asarone possesses hypocholesterolemic, hypolipidemic, neuroprotective, and antiepileptic activities [1016]. Methyl eugenol was also found to have antinociceptive, antibacterial, and antidepressive activities [17, 18]. These compounds were often used as reference standards for quality control of Acorus tatarinowii Schott due to outstanding pharmacological activities. The development of sensitive and rapid approach for simultaneous determination of these compounds was owing to increasing interest in the literature. High performance liquid chromatography (HPLC), gas chromatography (GC), and GC with mass spectrometric detection (MS) were the main assays for analyzing - and -asarone in the medicinal plant and plasma in previous studies [1923]. HPLC and MS method was also established for pharmacokinetics study of methyl eugenol in plasma [24, 25].

To our knowledge scope, few methods were found to be feasible for simultaneous determination of -asarone, -asarone, and methyl eugenol of Acorus tatarinowii Schott in biological samples, which produced issues of inaccurate evaluation of the medicinal plant and limitation of the pharmacokinetic study. This work was designed to develop a sensitive and valid method for determining -asarone, -asarone, and methyl eugenol of Acorus tatarinowii Schott in rat plasma. The work also aimed to apply the proposed assay in pharmacokinetic study of the three compounds after oral administration of volatile oil extracted from Acorus tatarinowii Schott.

2. Experimental

2.1. Chemicals and Reagents

-Asarone (>98%) and 1-naphthol (>99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methyl eugenol (>99%) and -asarone (>95%) were purchased from J&K Scientific Ltd (Guangdong, China). All other chemicals and reagents used in the experiment were of analytical grade unless stated specially. Acorus tatarinowii Schott was purchased from Xi’an Medical Company in China and identified by Professor Minfeng Fang in Northwest University.

The volatile oil of Acorus tatarinowii Schott was obtained by steam distillation of dry material of the plant. Acorus tatarinowii Schott (100.0 g) was put into 800 mL of distilled water and distilled for 8 h. The oil was collected from the condenser, dried by anhydrous sodium sulphate. The content of the oil in Acorus tatarinowii Schott was calculated to be 1.03% (v/w), with a determination of 0.50% methyl eugenol, 74.01% -asarone, and 11.41% -asarone by GC-MS analysis through area normalization method.

2.2. Preparation of Standard Solutions
2.2.1. Internal Standard

A stock solution of 1-naphthol (1.0 mg/mL) for IS was prepared in acetone and diluted to a concentration of 2.0 μg/mL with acetone.

2.2.2. Standard Solutions

Stock solution of -asarone, -asarone, and methyl eugenol was prepared by dissolving the appropriate amount of the standards in acetone. Mixed standard solution was prepared using the above solutions to give concentrations of 50.0 μg/mL for -asarone, 50.0 μg/mL for -asarone, and 50.0 μg/mL for methyl eugenol. All the solutions were stored at 4°C in amber glass tubes.

2.3. GC-MS/SIM Conditions

GC-MS analyses were performed with a Shimadzu GC-MS QP2010 (Kyoto, Japan). Chromatographic separation was achieved on a Restek Rxi-5MS capillary column (30 m × 0.32 mm I.D., 0.25 μm film thickness, Shimadzu, Japan) using nitrogen as carrier gas at 2 mL/min in a constant flow rate mode. The GC oven temperature was initially increased from 60°C to 120°C at a rate of 5°C/min, then elevated at a rate of at 2°C/min up to 150°C, and then raised to 240°C at a rate of 10°C/min, giving a total run time of 36 min. The temperatures of injector, interface, and ion source were 250, 280, and 230°C, respectively. Detection was operated by selected ionmonitoring (SIM) mode (70 eV, electron impact mode).

In the SIM mode, the peaks of -asarone, -asarone, methyl eugenol, and IS in plasma were identified by matching the retention time and the abundant ions of m/z 178 for methyl eugenol, m/z 208 for -asarone, -asarone, and m/z 144 for IS. The injection volume was 1.0 μL. Data were collected using the GC-MS solution (Kyoto, Japan).

2.4. Animals and Plasma Collection

Six healthy male Sprague-Dawley rats, weighing 250–280 g, were supplied by the Guangdong Laboratory Animals Monitoring Institute (Guangdong, China). The rats were housed in a standard animal holding room with normal access to food and water and 12 h light cycle. The animals were fasted overnight prior to dosing, with water allowance ad libitum. All animal experiments were carried out according to the Guidelines for the Care and Use of Laboratory Animals and were approved by the Animal Experimentation Ethics Committee.

Drug-free rat plasma samples were obtained from the postorbital venous plexus veins of anesthetized animals and collected into heparinized tubes. All the blood samples were centrifuged for 10 min at 8000 rpm to yield the supernatant stored at −20°C until analysis.

2.5. Sample Preparation

In a 1.5 mL Eppendorf tube, 100 μL of plasma was spiked with 10 μL of 2.0 μg/mL IS solution. An aliquot of 40 μL 10% (V : V) trichloracetic acid water solution and 600 μL acetonitrile was used to remove the proteins in the plasma through vortexed for 2 min followed by the centrifugation of 10 min at 8000 rpm. The supernatant was collected in a clean tube for next use, while the residue was extracted by 300 μL acetic ether for three times. The acetic ether layer and the above supernatant were collected together and evaporated to dryness under a stream of nitrogen. The residue was dissolved in 100 μL of acetone for further GC-MS analysis.

2.6. Method Validation
2.6.1. Calibration and Detection Limits

The linearity of -asarone, -asarone, and methyl eugenol was investigated by replicate analyses of calibration solutions with different concentration ranging from 5 to 5000 ng/mL and evaluated by linear regression. The limit of detection (LOD) was calculated by sequential diluting the analytes until the ratio of signal to noise equals 3 : 1. The limit of quantitation (LOQ) was considered as ten times of the signal to noise ratio.

2.6.2. Precision, Accuracy, and Stability

Quality control (QC) samples to determine the accuracy and precision of the method were independently prepared by dissolving the standard in plasma at low, medium, and high concentrations of 0.025, 0.25 and 2.5 μg/mL. The intraday precision and accuracy were determined within one day by analyzing the QC samples ( ). The interday precision and accuracy were determined on five separated days using the same QC samples. Precision was reported as the relative standard deviation (RSD). Accuracy was expressed as the relative error (RE).

The stability of analytes in the plasma was evaluated using the QC samples in triplicate. Test conditions included 4 freeze-thaw cycles and room temperature stability (0, 2, 4, 8, 12, and 24 h). The stabilities were assessed by analysis of the RSD of measured samples.

2.6.3. Recoveries and Matrix Effect

The extraction recovery of the three drugs was determined by calculating the peak areas obtained from blank plasma samples spiked with analyte before extraction with those from blank plasma samples, to which analytes were added after extraction. According to the guidance of USFDA, recovery experiments should be performed at three concentrations (low, medium, and high). This procedure was accordingly repeated for five replicates at three concentrations of 0.025, 0.25, and 2.5 μg/mL. In order to test the matrix effect on the ionization of the analyte, that is, the potential ion suppression or enhancement due to the matrix components, the drugs at three concentration levels were added to the extract of 0.1 mL of blank plasma, evaporated and reconstituted with 0.1 mL of acetone; the corresponding peak areas (A) were compared with those of the drugs standard solutions evaporated directly and reconstituted with the same solvent (B). The ratio (A/B × 100) % was used to evaluate the matrix effect. By the same method, the matrix effect of internal standard was also evaluated.

2.7. Pharmacokinetics Analysis

The GC-MS method was successfully applied in the pharmacokinetic studies of -asarone, -asarone, and methyl eugenol in rats. Volatile oil of Acorus tatarinowii Schott was administered orally to the rat in a single dose of 0.2 g/kg. Blood samples were collected in heparinized tube at 0, 5, 10, 20, 30, 60, 90, 120, 180, 300, 480, 720, 960, and 1200 min after dose. All the samples were extracted and analyzed under the proposed condition.

2.8. Data Analysis

The Microsoft Excel Program Drug and Statistics 2.0 (T.C.M. Shanghai, China) was employed to process and calculate the pharmacokinetic parameters. The parameters of the area under curve (AUC), the maximum plasma concentration ( max) and the corresponding time ( max), the half-life of absorption ( 1/2), and so forth were used to decrypt the pharmacokinetic properties of -asarone, -asarone, and methyl eugenol. Statistical analysis of the biological data was performed by the Student’s -test. All results were expressed as arithmetic mean ± standard deviation (SD).

3. Results and Discussion

3.1. Preparation of the Plasma Samples

In order to quantify drugs in a plasma sample, it is often necessary to disrupt the protein-drug binding for the production of the drugs-free form. Acetonitrile and trichloracetic acid were separately evaluated for extracting -asarone, -asarone, and methyl eugenol from plasma. When choosing the trichloracetic acid, the recoveries of -asarone and -asarone were less than 50%. When choosing the acetonitrile, the recoveries of -asarone and -asarone were near 80%. The recoveries of methyl eugenol and IS just have minor change. Interestingly, a mixture of acetonitrile and trichloracetic acid (10%) in ratios of 15 : 1 was found to be efficient to accomplish the extracting task. The recoveries of analytes were more than 90%. This solvent was accordingly used to prepare the plasma samples in the present work.

3.2. Calibration and Detection Limits

The linear regression analysis was constructed by plotting the peak area ratio of the three analytes to IS against the concentrations of -asarone, -asarone, and methyl eugenol in plasma samples, respectively, shown in Table 1. The regression equation of the curves and the correlation coefficients ( ) was calculated to be and for -asarone, and for -asarone, and and for methyl eugenol, using weighted least squares linear regression (the weighing factor was ). For each drug, six calibration curves containing six concentrations (5, 25, 50, 250, 500, 2500, and 5000 ng/mL) were linear within the concentration range of 5–5000 ng/mL. The CV of the three drugs at each level varied from 2.0 to 12.3. And the relative bias of -asarone, -asarone, and methyl eugenol from the theoretical value varied from −3.0 to 4.6. As a result, the calibration curves of the three compounds exhibited good linearity within the experimental range. The LOD for -asarone, -asarone, and methyl eugenol was calculated to be 1.9, 1.8, and 1.5 ng/mL. The LOQ of the tested drugs was 4.7, 4.9, and 4.2 ng/mL.

AnalytesCalibration curveaCorrelation coefficientLOD (ng/mL)LOQ (ng/mL)

Methyl eugenol 0.9991.5534.236
-Asarone 0.9971.8024.872
-Asarone 0.9991.6854.719

In the linear regression equation, is expressed as analytes concentration ( g/mL) and is expressed as peak area of analytes/IS.
3.3. Precision, Accuracy, and Stability

The data from QC samples were calculated to estimate the intraday and interday precision and accuracy of the method. The results are presented in Table 2. It was found that the intraday precision for low, mid, and high QC levels of -asarone was 7.08%, 3.24%, and 4.85%, respectively, and that of the interday analysis was 4.80%, 4.42%, and 7.99%, with an accuracy (RE) within −4.74 to 4.04%. The precision for -asarone of the QCs was determined as 4.84%, 6.34% plus 4.99% for intraday and 6.20%, 3.75%, and 6.01% for interday. The accuracy was found ranging from −6.03 to 2.93%. For methyl eugenol, the intraday precisions were 5.18%, 6.69%, and 4.11%, while the interday precisions were 8.10%, 7.68%, and 4.74%. The accuracy of methyl eugenol ranged between −7.62 and 5.15% for the three levels of QC samples. The precision and accuracy of the present method conform to the criteria for the analysis of biological samples according to the guidance of USFDA, where the precision (RSD) determined at each concentration level is required not to exceed 15%.

AnalytesAdded ( g/mL)Precision (RSD, %)Accuracy (RE, %)

Methyl eugenol0.0255.



The stabilities were demonstrated by analysis of the RSD of measured samples. The results indicated that analytes were stable at 2, 4, 8, 12, and 24 h at room temperature and during multiple freeze-thaw cycles (≥95.6%). The three compounds were believed to have good stability in 24 h at room temperature and during three freeze-thaw cycles.

3.4. Recoveries and Matrix Effect

The results are presented in Table 3. The recoveries of -asarone from rat plasma were , , and % at concentration levels of 0.025, 0.25, and 2.5 μg/mL, respectively, and the mean extraction recovery of IS was %. The values for recoveries of -asarone using the proposed method were , , and %. For methyl eugenol, the extraction recoveries were calculated to be , , and %. In terms of matrix effect, all the ratios (A/B × 100)% defined as in Section 2.6.3 were between 92.4 and 105.2%, which means no matrix effect for the three compounds and IS in using current method (Figure 1).

AnalyteConcentration (μg/mL)Matrix effects (%, )RSD (%)Extraction recovery (%, )RSD (%)

Methyl eugenol0.02593.8 ± 5.25.5105.2 ± 5.45.1
0.25094.6 ± ± 6.26.7
2.500105.2 ± 4.74.596.5 ± 4.04.1

-Asarone0.02592.4 ± 6.66.5102.9 ± 5.04.9
0.25098.6 ± 6.26.394.0 ± 6.06.4
2.500101.3 ± 3.83.895.1 ± 4.85.0

-Asarone0.02596.9 ± 4.44.599.2 ± 7.07.1
0.250100.6 ± 7.87.895.3 ± 3.13.3
2.500103.8 ± 6.26.0100.7 ± 4.94.9

I.S.2.094.1 ± 4.04.393.6 ± 3.18.6

3.5. Pharmacokinetics Analysis

The present method was applied to the pharmacokinetic investigations of -asarone, -asarone, and methyl eugenol following single oral dose of 0.2 g/kg volatile oil of Acorus tatarinowii Schott. Mean plasma drug concentration-time curve of the three compounds in single dose study was shown in Figure 2, and the calculating parameters were in Table 4. The results indicated that the plasma profile of methyl eugenol was confirmed to be one-compartment open models, while the pharmacokinetic behavior of -asarone and -asarone was in line with two-compartment open model. Nevertheless, after oral administration of the volatile oil, the max of the three drugs were calculated to be 0.021, 0.53, and 2.47 μg/mL, with the max values of 10.00, 11.25, and 13.75 min. The AUC were determined as 2.49, 56.67, and 407.52 mg/L· min, while the values of AUC were 2.13, 368.43, and 47.51 mg/L· min. The ratios of AUC versus AUC for the three drugs were all less than 120%, which indicated that the proposed blood collecting time was feasible to evaluate the pharmacokinetic behaviors of the three compounds.

Analytes   (min)   (min)   (min)   (mg/L·min)   (mg/L·min)CL (mL/min/kg)Vd (L/kg)   (min)   ( g/mL)

Methyl Eugenol67.66 ± 3.322.13 ± 0.272.49 ± 0.250.40 ± 0.0439.35 ± 4.0810.00 ± 0.000.021 ± 0.001
-Asarone54.39 ± 29.4669.30 ± 0.21368.43 ± 40.05407.52 ± 55.780.38 ± 0.05149.87 ± 90.7813.75 ± 11.092.47 ± 0.58
-Asarone58.92 ± 20.8069.28 ± 6.1247.51 ± 28.4956.67 ± 35.910.60 ± 0.4422.93 ± 16.1111.25 ± 6.290.53 ± 0.24

4. Conclusions

A novel GC-MS/SIM method for simultaneously qualitative and quantitative analysis of -asarone, -asarone, and methyl eugenolin rat plasma is described in this work. The method combines the excellent power of separation performed by GC to the high sensitivity of MS detection system operating in SIM mode and it has the properties of high specificity, precision, accuracy, and stability. The method has been confirmed to be successful in pharmacokinetics study of -asarone, -asarone and methyl eugenol in Acorus tatarinowii Schott, which probably provides an alternative to monitor the three drugs in preclinical study.

Conflict of Interests

The authors declare that there is no conflict of interests.


This work was supported by grants from the Program for Changjiang Scholars and Innovative Research Team in Universities of China (IRT1174) and the Eleventh Five-Year National Science and Technology Support Program of China (no. 2008BAI51B01).


  1. Y. Irie and W. M. Keung, “Rhizoma acori graminei and its active principles protect PC-12 cells from the toxic effect of amyloid-β peptide,” Brain Research, vol. 963, no. 1-2, pp. 282–289, 2003. View at: Publisher Site | Google Scholar
  2. B. Lee, Y. Choi, H. Kim et al., “Protective effects of methanol extract of Acori graminei rhizoma and Uncariae Ramulus et Uncus on ischemia-induced neuronal death and cognitive impairments in the rat,” Life Sciences, vol. 74, no. 4, pp. 435–450, 2003. View at: Publisher Site | Google Scholar
  3. J. Cho, C. H. Yang, C. G. Park, H. Lee, and Y. H. Kim, “Inhibition of excitotoxic neuronal cell death by total extracts from oriental medicines used for stroke treatment,” Yakhak Hoeji, vol. 44, no. 1, pp. 29–35, 2000. View at: Google Scholar
  4. P. C. Dandiya and M. K. Menon, “Actions of asarone on behavior, stress, and hyperpyrexia, and its interactions with central stimulants,” Journal of Pharmacology and Experimental Therapeutics, vol. 145, pp. 42–46, 1964. View at: Google Scholar
  5. M. T. Hsieh, W. H. Peng, C. R. Wu, and W. H. Wang, “The ameliorating effects of the cognitive-enhancing Chinese herbs on scopolamine-induced amnesia in rats,” Phytotherapy Research, vol. 14, no. 5, pp. 375–377, 2000. View at: Google Scholar
  6. J.-F. Liao, S.-Y. Huang, Y.-M. Jan, L.-L. Yu, and C.-F. Chen, “Central inhibitory effects of water extract of Acori graminei rhizoma in mice,” Journal of Ethnopharmacology, vol. 61, no. 3, pp. 185–193, 1998. View at: Publisher Site | Google Scholar
  7. S. B. Vohora, S. A. Shah, and P. C. Dandiya, “Central nervous system studies on an ethanol extract of Acorus calamus rhizomes,” Journal of Ethnopharmacology, vol. 28, no. 1, pp. 53–62, 1990. View at: Google Scholar
  8. B.-S. Koo, K.-S. Park, J.-H. Ha, J. H. Park, J.-C. Lim, and D.-U. Lee, “Inhibitory effects of the fragrance inhalation of essential oil from acorus gramineus on central nervous system,” Biological and Pharmaceutical Bulletin, vol. 26, no. 7, pp. 978–982, 2003. View at: Google Scholar
  9. G. Wei, Y.-Q. Fang, D.-H. Liu, and S.-F. Lin, “Study on GC-MS fingerprint analysis in rhizome of volatile oil of Acorus tatarinowii,” China Journal of Chinese Materia Medica, vol. 29, no. 8, pp. 764–768, 2004. View at: Google Scholar
  10. Q. D. Wu, Y. Q. Fang, Y. Z. Chen, Z. S. Kuan, S. Y. Wang, and Y. P. He, “Protective effects of volatile oil of Acorus tatarinowii schott and β-Asarone on cardiovascular system,” Traditional Chinese Drug Research and Clinical Pharmacology, vol. 16, no. 4, pp. 244–247, 2005. View at: Google Scholar
  11. J. Liu, C. Li, G. Xing et al., “Beta-asarone attenuates neuronal apoptosis induced by beta amyloid in rat hippocampus,” Yakugaku Zasshi, vol. 130, no. 5, pp. 737–746, 2010. View at: Publisher Site | Google Scholar
  12. Y.-Q. Fang, C. Shi, L. Liu, and R.-M. Fang, “Pharmacokinetics of beta-asarone in rabbit blood, hippocampus, cortex, brain stem, thalamus and cerebellum,” Die Pharmazie, vol. 67, no. 2, pp. 120–123, 2012. View at: Google Scholar
  13. L. Rodríguez-Páez, M. Juárez-Sanchez, J. Antúnez-Solís, I. Baeza, and C. Wong, “α-asarone inhibits HMG-CoA reductase, lowers serum LDL-cholesterol levels and reduces biliary CSI in hypercholesterolemic rats,” Phytomedicine, vol. 10, no. 5, pp. 397–404, 2003. View at: Publisher Site | Google Scholar
  14. L. Garduño, M. Salazar, S. Salazar et al., “Hypolipidaemic activity of α-asarone in mice,” Journal of Ethnopharmacology, vol. 55, no. 2, pp. 161–163, 1997. View at: Publisher Site | Google Scholar
  15. J. Cho, Y. Ho Kim, J.-Y. Kong, C. Ha Yang, and C. Gook Park, “Protection of cultured rat cortical neurons from excitotoxicity by asarone, a major essential oil component in the rhizomes of Acorus gramineus,” Life Sciences, vol. 71, no. 5, pp. 591–599, 2002. View at: Publisher Site | Google Scholar
  16. H. Chen, W. G. Li, X. B. Zhang et al., “Alpha-asarone from Acorus Gramineus alleviates epilepsy by modulating A-type gaba receptors,” Neuropharmacology, vol. 65, no. 5, pp. 1–11, 2013. View at: Google Scholar
  17. M. C. B. Norte, R. M. Cosentino, and C. A. Lazarini, “Effects of methyl-eugenol administration on behavioral models related to depression and anxiety, in rats,” Phytomedicine, vol. 12, no. 4, pp. 294–298, 2005. View at: Publisher Site | Google Scholar
  18. S. Yano, Y. Suzuki, M. Yuzurihara et al., “Antinociceptive effect of methyleugenol on formalin-induced hyperalgesia in mice,” European Journal of Pharmacology, vol. 553, no. 1–3, pp. 99–103, 2006. View at: Publisher Site | Google Scholar
  19. H. B. Wu and Y. Q. Fang, “Pharmacokinetics of β-asarone in rats,” Acta Pharmaceutica Sinica, vol. 39, no. 10, pp. 836–8383, 2004. View at: Google Scholar
  20. X. H. Ke, G. Wei, and Y. Q. Fang, “Assay of β-asarone and α-asarone in Rhizoma acori tatarinowii by HPLC,” Chinese Traditional Patent Medicine, vol. 24, no. 10, pp. 791–792, 2002. View at: Google Scholar
  21. L. P. Wei and M. H. Wu, “Pharmacokinetic study of β-asarone in volatile oil from Acorus tatarinowii in rabbits,” Chinese Traditional and Herbal Drugs, vol. 36, no. 1, pp. 48–51, 2002. View at: Google Scholar
  22. L. Liu and Y.-Q. Fang, “Analysis of the distribution of β-asarone in rat hippocampus, brainstem, cortex and cerebellum with gas chromatography-mass spectrometry (GC-MS),” Journal of Medicinal Plant Research, vol. 5, no. 9, pp. 1728–1734, 2011. View at: Google Scholar
  23. C. Deng, S. Lin, T. Huang, G. Duan, and X. Zhang, “Development of gas chromatography/mass spectrometry following headspace solid-phase microextraction for fast determination of asarones in plasma,” Rapid Communications in Mass Spectrometry, vol. 20, no. 14, pp. 2120–2126, 2006. View at: Publisher Site | Google Scholar
  24. S. W. Graves and S. Runyon, “Determination of methyleugenol in rodent plasma by high-performance liquid chromatography,” Journal of Chromatography B, vol. 663, no. 2, pp. 255–262, 1995. View at: Publisher Site | Google Scholar
  25. D. B. Barr, J. R. Barr, S. L. Bailey et al., “Levels of methyleugenol in a subset of adults in the general U.S. population as determined by high resolution mass spectrometry,” Environmental Health Perspectives, vol. 108, no. 4, pp. 323–328, 2000. View at: Google Scholar

Copyright © 2013 Xue Meng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

More related articles

 PDF Download Citation Citation
 Download other formatsMore
 Order printed copiesOrder

Related articles

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.