Research Article
Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-Catenin
Table 2
The reaction system of q-PCR (
).
| | Composition | Plus the amount |
| | IQ SYBR Green Supermix | 10 μL | | Forward primer | 1 μL | | Reverse primer | 1 μL | | cDNA | 2 μL | | Nase-free water | 6 μL |
| | Total volume | 20 μL |
|
|
