Research Article
Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS+ System
Table 1
LC-MS operating condition.
| Instrument | Condition |
| Column | HECpTOR-M-RS-tech C18 (100 × 4.6 mm, 5 m) | Oven temp. (°C) | 40 | Flow rate (mL/min) | 0.5 | Injection vol. (L) | 10 | Ionization source (positive mode) | API-ES | Fragmentor voltage (V) | 70 | Quadrupole temp. (°C) | 99 | Capillary voltage (V) | 3000 | Nebulizer pressure (psi) | 35 | Drying gas temp. (°C) | 350 | Mass rang () scan mode | 200~500 | Mobile phase (%) | A: 0.1% TFA in water, B: acetonitrile | Gradient elution composition (%) | (B): 10–25 (0–35 min), 25–10 (35–37 min), 10 (37–45 min) |
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