Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study

Figure 2

Chromatograms of blank plasma (a); blank plasma spiked with (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate 20 μL (0.143 μg·mL⁻¹) and IS 20 μL (0.00504 mg⋅mL⁻¹) (b); rat plasma sample (4 h) after oral administration of raw Atractylodis Rhizoma 40 g·kg⁻¹ (c); rat plasma sample (4 h) after oral administration of processed Atractylodis Rhizoma 40 g⋅kg⁻¹ (d); Chromatograms of blank tissue homogenate (e); blank tissue homogenate with (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate 20 μL (0.286 μg·mL⁻¹) and IS 20 μL (0.00504 mg·mL⁻¹) (f); spleen sample (2 h) after oral administration of raw Atractylodis Rhizoma 40 g·kg⁻¹ (g); spleen sample (2 h) after oral administration of processed Atractylodis Rhizoma 40 g·kg⁻¹ (h).

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