Research Article

Determination of β-Cyano-L-alanine, γ-Glutamyl-β-cyano-L-alanine, and Common Free Amino Acids in Vicia sativa (Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography

Figure 1

HPLC analysis of the DEEMM derivatives of GCA, BCA, and amino acid standards (a) and seed extracts (b). 1 = Asp; 2 = Glu; 3 = GCA; 4 = Asn; 5 = Ser; 6 = Gln; 7 = His; 8 = Gly; 9 = Thr; 10 = Arg; 11 = Ala; 12 = BCA; 13 = Pro; 14 = I.S.; 15 = Tyr; 16 = ammonium ion; 17 = Val; 18 = Met; 19 = Cys-Cys; 20 = Ile; 21 = Trp; 22 = Leu; 23 = Phe; 24 = Cys; 25 = Lys.
(a)
(b)