Research Article

Determination of β-Cyano-L-alanine, γ-Glutamyl-β-cyano-L-alanine, and Common Free Amino Acids in Vicia sativa (Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography

Table 3

Recovery (mean and RSD) of the HPLC method for determination of GCA and BCA in V. sativa (sample 7 of Table 4).

Compound Initial content (μM) First concentration added (10 μM) Second concentration added (30 μM)Third concentration added (90 μM)
Content I (µM)RecoveryContent II (µM)RecoveryContent III (µM)Recovery

GCA 37.83 47.81 99.83 (0.76) 67.82 99.97 (0.16) 127.67 99.82 (0.33)
BCA 0.61 10.58 99.67 (0.55) 30.56 99.82 (0.20) 90.53 99.91 (0.09)