Determination of β-Cyano-L-alanine, γ-Glutamyl-β-cyano-L-alanine, and Common Free Amino Acids in Vicia sativa (Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography

Table 4

Contents (% w/w of dry weight) of GCA, BCA, and free amino acids in V. sativa seeds. Data are the mean of three determinations ± standard deviation.

Compound/sample	1	2	3	4	5	6	7	8
GCA	0.887 ± 0.008	1.071 ± 0.021	0.572 ± 0.015	1.029 ± 0.031	1.134 ± 0.020	0.946 ± 0.027	1.252 ± 0.018	1.010 ± 0.075
BCA	0.003 ± 0.000	0.005 ± 0.000	0.006 ± 0.000	0.017 ± 0.001	0.022 ± 0.001	0.007 ± 0.001	0.009 ± 0.000	0.003 ± 0.000
Asp	0.094 ± 0.002	0.029 ± 0.002	0.016 ± 0.001	0.013 ± 0.001	0.029 ± 0.000	0.013 ± 0.001	0.031 ± 0.002	0.027 ± 0.001
Glu	0.036 ± 0.000	0.058 ± 0.002	0.081 ± 0.002	0.083 ± 0.003	0.082 ± 0.005	0.040 ± 0.001	0.099 ± 0.003	0.066 ± 0.000
Asn	0.010 ± 0.001	0.031 ± 0.003	0.189 ± 0.003	0.045 ± 0.002	0.045 ± 0.000	0.021 ± 0.002	0.048 ± 0.004	0.046 ± 0.002
Ser	0.010 ± 0.001	0.007 ± 0.000	0.006 ± 0.000	0.005 ± 0.000	0.006 ± 0.000	0.005 ± 0.000	0.007 ± 0.001	0.006 ± 0.000
Gln	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000
His	0.009 ± 0.001	0.006 ± 0.000	0.037 ± 0.001	0.003 ± 0.000	0.002 ± 0.000	0.014 ± 0.001	0.013 ± 0.002	0.013 ± 0.001
Gly	0.009 ± 0.000	0.007 ± 0.000	0.007 ± 0.000	0.006 ± 0.001	0.006 ± 0.000	0.001 ± 0.000	0.005 ± 0.001	0.002 ± 0.000
Thr	0.065 ± 0.005	0.140 ± 0.003	0.014 ± 0.001	0.071 ± 0.003	0.028 ± 0.002	0.005 ± 0.001	0.007 ± 0.001	0.012 ± 0.001
Arg	0.072 ± 0.001	0.098 ± 0.009	0.301 ± 0.012	0.074 ± 0.012	0.157 ± 0.017	0.059 ± 0.002	0.124 ± 0.006	0.026 ± 0.003
Ala	0.007 ± 0.001	0.008 ± 0.001	0.014 ± 0.001	0.009 ± 0.001	0.014 ± 0.001	0.010 ± 0.001	0.012 ± 0.002	0.010 ± 0.001
Pro	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000
Try	0.003 ± 0.000	0.002 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.002 ± 0.000	0.002 ± 0.000	0.003 ± 0.001	0.002 ± 0.000
Val	0.009 ± 0.001	0.007 ± 0.001	0.007 ± 0.000	0.013 ± 0.001	0.004 ± 0.000	0.003 ± 0.000	0.003 ± 0.001	0.004 ± 0.001
Met	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000
Cys-Cys	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000
Ile	0.047 ± 0.001	0.002 ± 0.000	0.005 ± 0.000	0.005 ± 0.000	0.000 ± 0.000	0.001 ± 0.000	0.009 ± 0.000	0.009 ± 0.001
Trp	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000
Leu	0.011 ± 0.001	0.014 ± 0.000	0.020 ± 0.001	0.007 ± 0.000	0.011 ± 0.000	0.002 ± 0.000	0.007 ± 0.000	0.019 ± 0.001
Phe	0.003 ± 0.000	0.003 ± 0.000	0.007 ± 0.001	0.005 ± 0.000	0.007 ± 0.000	0.008 ± 0.000	0.004 ± 0.000	0.008 ± 0.000
Cys	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000	0.000 ± 0.000
Lys	0.003 ± 0.000	0.003 ± 0.000	0.004 ± 0.001	0.003 ± 0.000	0.004 ± 0.000	0.004 ± 0.001	0.004 ± 0.000	0.002 ± 0.000