Quantification of Neurotransmitters in Mouse Brain Tissue by Using Liquid Chromatography Coupled Electrospray Tandem Mass Spectrometry
Table 1
The calibration of neurotransmitters by LC-MS/MS.
Chemical
Internal standard
Equationsa
Linear range (ng/g)
Correlation coefficient ()
LOD (ng/g)
LOQ (ng/g)
BH4
AACA
= 2.89 10−5 − 2.8 10−4
10–10000
0.9964
1
10
Dopamine
Dopamine-D4
= 5.03 10−5 − 2.310−5
10–10000
0.9967
1
10
Serotonin
Serotonin-D4
= 9.92 10−5 − 1.69 10−4
20–10000
0.9940
2
20
Norepinephrine
Norepinephrine-D6
= 1.20 10−4 + 9.3 10−3
20–10000
0.9927
2
20
Epinephrine
Epinephrine-D3
= 8.00 10−5 + 2.86 10−4
20–10000
0.9929
2
20
Glutamate
Glutamate-D5
= 0.1409 + 1.16 10−2
200–200,000
0.9964
20
200
GABA
GABA-D6
= 0.0453 − 1.57 10−4
200–200,000
0.9986
20
200
LOD; limit of detection. LOQ; limit of quantitation. The calibration curves were constructed by plotting the peak area ratio to IS versus the concentration of each analyte.
