Research Article

Quantification of Neurotransmitters in Mouse Brain Tissue by Using Liquid Chromatography Coupled Electrospray Tandem Mass Spectrometry

Table 4

The levels of tetrahydrobiopterin (BH4) and neurotransmitters in mouse brain regions.

Brain regionsBH4 (ng/g)DA (ng/g)5-HT (ng/g)NE (ng/g)EP (ng/g)Glu (g/g)GABA (g/g)

Striatum164.0 ± 21.793463 ± 200.9207.7 ± 17.81457.6 ± 73.5928.55 ± 3.061037 ± 86.57397.4 ± 7.466
Midbrain129.2 ± 9.8659.8 ± 6.19486.7 ± 50.10802.5 ± 60.5273.5 ± 9.54670.2 ± 98.11682.2 ± 34.66
Hippocampus21.5 ± 1.40ND98.1 ± 13.643170 ± 669.1281.3 ± 33.373507 ± 1431721.0 ± 56.15
Olfactory bulb577.2 ± 59.7940.6 ± 7.3484.93 ± 9.88734.4 ± 95.5359.7 ± 14.59597.8 ± 90.36772.1 ± 92.21
Frontal cortex212.5 ± 52.27ND81.83 ± 6.25320.1 ± 55.3044.9 ± 6.851090 ± 108.1430.8 ± 17.47
Hypothalamus84.8 ± 14.17111.9 ± 26.17282.4 ± 28.422627 ± 135.4188.0 ± 27.68811.8 ± 136.8829.7 ± 65.56
Cerebellum293.4 ± 69.74ND29.17 ± 4.07584.5 ± 79.12ND791.9 ± 62.29389.9 ± 14.88
Brainstem60.9 ± 9.02ND452.5 ± 50.031123 ± 82.1178.4 ± 9.71622.5 ± 37.00348.9 ± 18.10
Pituitary gland92.2 ± 19.2948.9 ± 18.9ND2539 ± 358.535.5 ± 9.00536.6 ± 70.2522.03 ± 1.733

Unit: Mean ± SEM ug/tissue weight (g).