Table of Contents Author Guidelines Submit a Manuscript
Journal of Analytical Methods in Chemistry
Volume 2014, Article ID 563702, 9 pages
http://dx.doi.org/10.1155/2014/563702
Research Article

Extraction and Bioactivity Analysis of Major Flavones Compounds from Scutellaria baicalensis Using In Vitro Assay and Online Screening HPLC-ABTS System

KM-Based Herbal Drug Development Group, Korean Institute of Oriental Medicine (KIOM), 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea

Received 1 July 2014; Revised 18 August 2014; Accepted 18 August 2014; Published 1 September 2014

Academic Editor: Hassan Y. Aboul Enein

Copyright © 2014 Kwang Jin Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. K. Ishimaru, K. Nishikawa, T. Omoto, I. Asai, K. Yoshihira, and K. Shimomura, “Two flavone 2′-glucosides from Scutellaria baicalensis,” Phytochemistry, vol. 40, no. 1, pp. 279–281, 1995. View at Publisher · View at Google Scholar · View at Scopus
  2. H. G. Park, S. Y. Yoon, J. Y. Choi et al., “Anticonvulsant effect of wogonin isolated from Scutellaria baicalensis,” European Journal of Pharmacology, vol. 574, no. 2-3, pp. 112–119, 2007. View at Publisher · View at Google Scholar · View at Scopus
  3. H. Cao, Y. Jiang, J. Chen et al., “Arsenic accumulation in Scutellaria baicalensis Georgi and its effects on plant growth and pharmaceutical components,” Journal of Hazardous Materials, vol. 171, no. 1–3, pp. 508–513, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. C. Zhang, Y. Zhang, J. Chen, and X. Liang, “Purification and characterization of baicalin-β-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge,” Process Biochemistry, vol. 40, no. 5, pp. 1911–1915, 2005. View at Publisher · View at Google Scholar · View at Scopus
  5. M. Himeji, T. Ohtsuki, H. Fukazawa et al., “Difference of growth-inhibitory effect of Scutellaria baicalensis-producing flavonoid wogonin among human cancer cells and normal diploid cell,” Cancer Letters, vol. 245, no. 1-2, pp. 269–274, 2007. View at Publisher · View at Google Scholar · View at Scopus
  6. Z. Gao, K. Huang, X. Yang, and H. Xu, “Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi,” Biochimica et Biophysica Acta—General Subjects, vol. 1472, no. 3, pp. 643–650, 1999. View at Publisher · View at Google Scholar · View at Scopus
  7. C. Li, L. Zhang, G. Lin, and Z. Zuo, “Identification and quantification of baicalein, wogonin, oroxylin A and their major glucuronide conjugated metabolites in rat plasma after oral administration of Radix scutellariae product,” Journal of Pharmaceutical and Biomedical Analysis, vol. 54, no. 4, pp. 750–758, 2011. View at Publisher · View at Google Scholar · View at Scopus
  8. H.-B. Li and F. Chen, “Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography,” Journal of Chromatography A, vol. 1074, no. 1-2, pp. 107–110, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. M.-C. Lin, M.-J. Tsai, and K.-C. Wen, “Supercritical fluid extraction of flavonoids from Scutellariae Radix,” Journal of Chromatography A, vol. 830, no. 2, pp. 387–395, 1999. View at Publisher · View at Google Scholar · View at Scopus
  10. Y. Jin, Y. S. Kim, Y. D. Cheng, and K. J. Lee, “Extraction of kurarinone and leachianone A from Sophora flavescens ait using ultrasonic wave,” Asian Journal of Chemistry, vol. 24, no. 7, pp. 2917–2920, 2012. View at Google Scholar · View at Scopus
  11. H. Wang, L. Chen, Y. Xu et al., “Dynamic microwave-assisted extraction coupled on-line with clean-up for determination of caffeine in tea,” LWT—Food Science and Technology, vol. 44, no. 6, pp. 1490–1495, 2011. View at Publisher · View at Google Scholar · View at Scopus
  12. K. J. Lee, S. D. Choi, and J. Y. Ma, “Phytochemical analysis of curcumin from turmeric by RP-HPLC,” Asian Journal of Chemistry, vol. 25, no. 2, pp. 995–998, 2013. View at Google Scholar · View at Scopus
  13. Y.-J. Li, J. Chen, and P. Li, “Identification and quantification of free radical scavengers in the flower buds of Lonicera species by online HPLC-DPPH assay coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry,” Biomedical Chromatography, vol. 26, no. 4, pp. 449–457, 2012. View at Publisher · View at Google Scholar · View at Scopus
  14. H. A. G. Niederländer, T. A. van Beek, A. Bartasiute, and I. I. Koleva, “Antioxidant activity assays on-line with liquid chromatography,” Journal of Chromatography A, vol. 1210, no. 2, pp. 121–134, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. N. Pellegrini, M. Ying, and C. Rice-Evans, “Screening of dietary carotenoids and carotenoid-rich fruits extract for antioxidant activities applying 2, 2′-azobis (3-ethylbenzothine-6-surfonic acid) radical cation decolorization assay,” Methods in Enzymology, vol. 299, pp. 384–389, 1999. View at Google Scholar
  16. A. J. Stewart, W. Mullen, and A. Crozier, “On-line high-performance liquid chromatography analysis of the antioxidant activity of phenolic compounds in green and black tea,” Molecular Nutrition and Food Research, vol. 49, no. 1, pp. 52–60, 2005. View at Publisher · View at Google Scholar · View at Scopus
  17. H.-J. Choi, O.-H. Kang, P.-S. Park et al., “Mume Fructus water extract inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated macrophages,” Journal of Medicinal Food, vol. 10, no. 3, pp. 460–466, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. Y.-C. Oh, W.-K. Cho, Y. H. Jeong et al., “Anti-inflammatory effect of Sosihotang via inhibition of nuclear factor-κB and mitogen-activated protein kinases signaling pathways in lipopolysaccharide-stimulated RAW 264.7 macrophage cells,” Food and Chemical Toxicology, vol. 53, pp. 343–351, 2013. View at Publisher · View at Google Scholar · View at Scopus
  19. J.-H. Park, R.-Y. Kim, and E. Park, “Antioxidant and α-glucosidase inhibitory activities of different solvent extracts of skullcap (Scutellaria baicalensis),” Food Science and Biotechnology, vol. 20, no. 4, pp. 1107–1112, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. L.-L. Dong, Y.-J. Fu, Y.-G. Zu et al., “An enhanced preparation and purification of the major antioxidants baicalein and wogonin from Scutellariae radix,” Food Chemistry, vol. 133, no. 2, pp. 430–436, 2012. View at Publisher · View at Google Scholar · View at Scopus
  21. C. Li, L. Zhou, G. Lin, and Z. Zuo, “Contents of major bioactive flavones in proprietary traditional Chinese medicine products and reference herb of Radix Scutellariae,” Journal of Pharmaceutical and Biomedical Analysis, vol. 50, no. 3, pp. 298–306, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. H.-B. Li, Y. Jiang, and F. Chen, “Separation methods used for Scutellaria baicalensis active components,” Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 812, no. 1-2, pp. 277–290, 2004. View at Publisher · View at Google Scholar · View at Scopus